| Title: | "Cloning, monoclonal antibody production, and bodily distribution pattern of a bovine lipocalin" |
| Author(s): | Japaridze T; Senda A; Nozaki H; Yanagida M; Suzuki T; Ganzorig K; Kushi Y; Kida K; Urashima T; Bruckmaier RM; Fukuda K; |
| Address: | "Department of Animal and Food Hygiene, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido, Japan" |
| Journal Title: | Biosci Biotechnol Biochem |
| ISSN/ISBN: | 1347-6947 (Electronic) 0916-8451 (Linking) |
| Abstract: | "A bovine lipocalin, previously identified as a putative odorant-binding protein in bovine colostrum (bcOBP), was cloned and expressed, and its monoclonal antibody was established. bcOBP was constantly secreted into milk on day of parturition until at least 10 d postpartum at a concentration of 181+/-39 microg/L. Besides milk, bcOBP occurred in the nasal mucus, saliva, amniotic fluid, vaginal discharge, and blood plasma. Despite its low concentration, the distribution pattern and the finding that bcOBP harbored a characteristic sequence motif, CxxxC, which is conserved among insect and mammal pheromone binding proteins, suggest that bcOBP functions as a pheromone carrier. The presence of bcOBP in the plasma at varied concentrations depending on the lactation period does not exclude the possibility that bcOBP is secreted into milk from the blood. Cross-reactivity of the monoclonal antibody indicated presence of proteins homologous to bcOBP in the colostrum of farm animals of Cetartiodactyla" |
| Keywords: | "Amino Acid Motifs Animals Antibodies, Monoclonal/*biosynthesis Body Fluids/chemistry Cattle/*metabolism Cloning, Molecular Colostrum/*chemistry Female Lactation/physiology Lipocalins/*chemistry/genetics/metabolism Mice Mice, Inbred BALB C Milk/chemistry M;" |
| Notes: | "MedlineJaparidze, Tamar Senda, Akitsugu Nozaki, Hirofumi Yanagida, Mayumi Suzuki, Takumi Ganzorig, Khuukhenbaatar Kushi, Yasunori Kida, Katsuya Urashima, Tadasu Bruckmaier, Rupert M Fukuda, Kenji eng Research Support, Non-U.S. Gov't England 2012/04/10 Biosci Biotechnol Biochem. 2012; 76(4):712-20. doi: 10.1271/bbb.110840. Epub 2012 Apr 7" |
