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J Med Entomol

Title:		Evaluation of extracts and oils of mosquito (Diptera: Culicidae) repellent plants from Sweden and Guinea-Bissau
Author(s):		Jaenson TG; Palsson K; Borg-Karlson AK;
Address:		"Medical Entomology Unit, Department of Systematic Zoology, Evolutionary Biology Centre, Uppsala University, Norbyvagen 18 d, SE-752 36 Uppsala, Sweden"
Journal Title:		J Med Entomol
Year:		2006
Volume:		43
Issue:		1
Page Number:		113 - 119
DOI:		10.1093/jmedent/43.1.113
ISSN/ISBN:		0022-2585 (Print) 0022-2585 (Linking)
Abstract:		"In laboratory tests, ethyl acetate extracts of Hyptis suaveolens Poit. from Guinea-Bissau and Rhododendon tomentosum (Stokes) H. Harmaja (formerly Ledum palustre L.) and Myrica gale L. significantly reduced probing activity of Aedes aegypti (L.). In the field in southern Sweden, extracts of leaves of R. tomentosum, M. gale, and Achillea millefolium L. significantly reduced biting by Aedes mosquitoes. Volatile compounds from M. gale, R. tomentosum, A. millefolium, and H. suaveolens were collected by solid phase microextraction (SPME). Alternatively, compounds in the plants were subjected to extraction by organic solvents of different polarities or by steam distillation and collection by SPME. Compounds collected were identified by gas chromatography-mass spectrometry. Leaves of H. suaveolens contained mainly beta-caryophyllene, bergamotene, and terpinolene. The volatile fraction of an ethyl acetate extract of H. suaveolens was collected by SPME and included beta-caryophyllene, (-) -sabinene, beta-pinene, limonene, alpha-pinene, and bergamotene. The main volatiles detected were alpha-pinene, alpha-phellandrene, myrcene, and limonene from M. gale leaves or inflorescences; pcymene, sabinene, and terpinyl acetate from leaves of R. tomentosum; and (-)-germacrene D, beta-pinene, sabinene, and alpha-pinene from A. millefolium leaves or inflorescences. The selected plant species contained numerous volatiles known to have insecticidal, acaricidal, 'pesticidal,' and/ or insect repellent properties"
Keywords:		Acetates/chemistry Achillea/chemistry Aedes/*drug effects Animals Biological Assay Female Gas Chromatography-Mass Spectrometry/methods Guinea-Bissau Humans Hyptis/chemistry Insect Bites and Stings/prevention & control Insect Repellents/chemistry/isolation;
Notes:		"MedlineJaenson, Thomas G T Palsson, Katinka Borg-Karlson, Anna-Karin eng Research Support, Non-U.S. Gov't England 2006/03/02 J Med Entomol. 2006 Jan; 43(1):113-9. doi: 10.1093/jmedent/43.1.113"