| Title: | "Isolation and characterization of calmodulin in the pheromone gland of the silkworm, Bombyx mori" |
| Author(s): | Iwanaga M; Dohmae N; Fonagy A; Takio K; Kawasaki H; Maeda S; Matsumoto S; |
| Address: | "Molecular Entomology and Baculovirology, The Institute of Physical and Chemical Research (RIKEN), Saitama, Japan" |
| Journal Title: | Comp Biochem Physiol B Biochem Mol Biol |
| DOI: | 10.1016/s0305-0491(98)10076-7 |
| ISSN/ISBN: | 1096-4959 (Print) 1096-4959 (Linking) |
| Abstract: | "Production of the sex pheromone bombykol in the silkworm, Bombyx mori, is regulated by a neurohormone termed pheromone biosynthesis activating neuropeptide (PBAN). It has been suggested that the external signal of PBAN in this species is transmitted to the intracellular cascade reactions consisting of Ca2+/calmodulin (CaM) complex and phosphoprotein phosphatase. To demonstrate the molecular mechanisms regulated by PBAN, we attempted to characterize CaM in the pheromone gland of B. mori. By using ion-exchange and RP-HPLC, B. mori CaM was purified from the cytosolic fraction of the pheromone gland. The primary structure was deduced by composition/sequence analysis and mass spectrometric analysis of the fragment peptides obtained from enzymatic and chemical fragmentations. The amino acid sequence of B. mori CaM was identical with Drosophila CaM deduced from the CaM gene of D. melanogaster, suggesting that insects have well conserved the molecule of CaM" |
| Keywords: | Amino Acid Sequence Amino Acids/analysis Animals Bombyx/genetics/*metabolism Calmodulin/genetics/*isolation & purification/metabolism Female Male Molecular Sequence Data Neuropeptides/metabolism Peptide Mapping Sex Attractants/biosynthesis Signal Transduc; |
| Notes: | "MedlineIwanaga, M Dohmae, N Fonagy, A Takio, K Kawasaki, H Maeda, S Matsumoto, S eng Research Support, Non-U.S. Gov't England 1998/12/17 Comp Biochem Physiol B Biochem Mol Biol. 1998 Aug; 120(4):761-7. doi: 10.1016/s0305-0491(98)10076-7" |
