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Methods Mol Biol

Title:		Genetic manipulation to analyze pheromone responses: knockouts of multiple receptor genes
Author(s):		Ishii T;
Address:		"Department of Cell Biology, Graduate School of Medical and Dental Science, Tokyo Medical and Dental University, Tokyo, Japan"
Journal Title:		Methods Mol Biol
Year:		2013
Volume:		1068
Issue:
Page Number:		133 - 154
DOI:		10.1007/978-1-62703-619-1_10
ISSN/ISBN:		1940-6029 (Electronic) 1064-3745 (Linking)
Abstract:		"Gene targeting in the mouse is an essential technique to study gene function in vivo. Multigene families encoding vomeronasal receptor (VR) type 1 and type 2 consist of ~300 intact genes, which are clustered at multiple loci in the mouse genome. To understand the function of VRs and neurons expressing a particular VR in vivo, individual endogenous receptor genes can be manipulated by conventional gene targeting to create loss-of-function mutations or to visualize neurons and their axons expressing the VR. Multiple receptor genes in a cluster can also be deleted simultaneously by chromosome engineering, allowing analysis of function of a particular VR subfamily. Here, we describe protocols for conventional gene targeting and chromosome engineering for deleting a large genomic region in mouse embryonic stem (ES) cells"
Keywords:		"Animals Cells, Cultured Embryonic Stem Cells/cytology Gene Targeting Mice Mice, Inbred C57BL Mice, Knockout Multigene Family/genetics Receptors, G-Protein-Coupled/*analysis/chemistry/genetics Receptors, Pheromone/*analysis/chemistry/genetics Sequence Dele;"
Notes:		"MedlineIshii, Tomohiro eng Research Support, Non-U.S. Gov't 2013/09/10 Methods Mol Biol. 2013; 1068:133-54. doi: 10.1007/978-1-62703-619-1_10"