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ACS Sens

Title:		Fiber-Optic Bio-sniffer (Biochemical Gas Sensor) Using Reverse Reaction of Alcohol Dehydrogenase for Exhaled Acetaldehyde
Author(s):		Iitani K; Chien PJ; Suzuki T; Toma K; Arakawa T; Iwasaki Y; Mitsubayashi K;
Address:		"Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University , 1-5-45 Yushima, Bunkyo-ku, Tokyo 113-8510, Japan. Department of Biomedical Devices and Instrumentation, Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University ,2-3-10 Kanda-Surugadai, Chiyoda-ku, Tokyo 101-0062, Japan. Faculty of Chemistry, Materials and Bioengineering, Kansai University , 3-3-35 Yamate-Cho, Suita-Shi, Osaka 564-0836, Japan"
Journal Title:		ACS Sens
Year:		2018
Volume:		20180213
Issue:		2
Page Number:		425 - 431
DOI:		10.1021/acssensors.7b00865
ISSN/ISBN:		2379-3694 (Electronic) 2379-3694 (Linking)
Abstract:		"Volatile organic compounds (VOCs) exhaled in breath have huge potential as indicators of diseases and metabolisms. Application of breath analysis for disease screening and metabolism assessment is expected since breath samples can be noninvasively collected and measured. In this research, a highly sensitive and selective biochemical gas sensor (bio-sniffer) for gaseous acetaldehyde (AcH) was developed. In the AcH bio-sniffer, a reverse reaction of alcohol dehydrogenase (ADH) was employed for reducing AcH to ethanol and simultaneously consuming a coenzyme, reduced form of nicotinamide adenine dinucleotide (NADH). The concentration of AcH can be quantified by fluorescence detection of NADH that was consumed by reverse reaction of ADH. The AcH bio-sniffer was composed of an ultraviolet light-emitting diode (UV-LED) as an excitation light source, a photomultiplier tube (PMT) as a fluorescence detector, and an optical fiber probe, and these three components were connected with a bifurcated optical fiber. A gas-sensing region of the fiber probe was developed with a flow-cell and an ADH-immobilized membrane. In the experiment, after optimization of the enzyme reaction conditions, the selectivity and dynamic range of the AcH bio-sniffer were investigated. The AcH bio-sniffer showed a short measurement time (within 2 min) and a broad dynamic range for determination of gaseous AcH, 0.02-10 ppm, which encompassed a typical AcH concentration in exhaled breath (1.2-6.0 ppm). Also, the AcH bio-sniffer exhibited a high selectivity to gaseous AcH based on the specificity of ADH. The sensor outputs were observed only from AcH-contained standard gaseous samples. Finally, the AcH bio-sniffer was applied to measure the concentration of AcH in exhaled breath from healthy subjects after ingestion of alcohol. As a result, a significant difference of AcH concentration between subjects with different aldehyde dehydrogenase type 2 (ALDH2) phenotypes was observed. The AcH bio-sniffer can be used for breath measurement, and further, an application of breath analysis-based disease screening or metabolism assessment can be expected due to the versatility of its detection principle, which allows it to measure other VOCs by using NADH-dependent dehydrogenases"
Keywords:		"Acetaldehyde/*analysis Alcohol Dehydrogenase/*chemistry Alcohol Drinking Biosensing Techniques/*methods Breath Tests Enzymes, Immobilized/*chemistry Exhalation Fiber Optic Technology/*methods Humans Saccharomyces cerevisiae/enzymology Sensitivity and Spec;"
Notes:		"MedlineIitani, Kenta Chien, Po-Jen Suzuki, Takuma Toma, Koji Arakawa, Takahiro Iwasaki, Yasuhiko Mitsubayashi, Kohji eng Research Support, Non-U.S. Gov't 2018/01/31 ACS Sens. 2018 Feb 23; 3(2):425-431. doi: 10.1021/acssensors.7b00865. Epub 2018 Feb 13"