| Title: | Highly sensitive olfactory biosensors for the detection of volatile organic compounds by surface plasmon resonance imaging |
| Author(s): | Hurot C; Brenet S; Buhot A; Barou E; Belloir C; Briand L; Hou Y; |
| Address: | "Uni. Grenoble Alpes, CEA, CNRS, INAC-SyMMES, 17 Rue des Martyrs, 38000 Grenoble, France. INRA, CNRS, Universite de Bourgogne - Franche Comte, Centre des Sciences du Gout et de l'Alimentation, 17 rue Sully, 21000 Dijon, France. Uni. Grenoble Alpes, CEA, CNRS, INAC-SyMMES, 17 Rue des Martyrs, 38000 Grenoble, France. Electronic address: yanxia.hou-broutin@cea.fr" |
| DOI: | 10.1016/j.bios.2018.08.072 |
| ISSN/ISBN: | 1873-4235 (Electronic) 0956-5663 (Linking) |
| Abstract: | "Nowadays, monitoring of volatile organic compounds (VOCs) is very important in various domains. In this work, we aimed to develop sensitive olfactory biosensors using odorant binding proteins (OBPs) as sensing materials. Three rat OBP3 derivatives with customized binding properties were designed and immobilized on the same chip for the detection of VOCs in solution by surface plasmon resonance imaging (SPRi). We demonstrated that the proteins kept their binding properties after the immobilization under optimized conditions. The obtained olfactory biosensors exhibited very low limits of detection in both concentration (200?ª+pM of beta-ionone) and in molecular weight of VOCs (100?ª+g/mol for hexanal). Such a performance obtained with SPRi in solution is especially remarkable. We hypothesized that the binding of VOCs to the active sites of OBPs induced a local conformational change in the proteins. This change would??? give rise to a variation of refractive index, to which SPRi is extremely sensitive. In addition, the olfactory biosensors showed a high selectivity especially at relatively low VOC concentrations. With optimized regeneration procedures, they also showed very good repeatability not only from measurement to measurement but also from chip to chip with a lifespan up to almost two months. These olfactory biosensors are particularly interesting for trace detection of VOCs in solution" |
| Keywords: | "Animals *Biosensing Techniques Rats Receptors, Odorant/*chemistry *Smell Surface Plasmon Resonance Volatile Organic Compounds/chemistry/*isolation & purification Odorant binding proteins Olfactory biosensor Surface plasmon resonance imaging Volatile organ;" |
| Notes: | "MedlineHurot, Charlotte Brenet, Sophie Buhot, Arnaud Barou, Emilie Belloir, Christine Briand, Loic Hou, Yanxia eng England 2018/09/12 Biosens Bioelectron. 2019 Jan 1; 123:230-236. doi: 10.1016/j.bios.2018.08.072. Epub 2018 Aug 31" |
