| Title: | Determination of indole alkaloids and highly volatile compounds in Rauvolfia verticillata by HPLC-UV and GC-MS |
| Author(s): | Hong B; Li W; Song A; Zhao C; |
| Address: | "1Institute of Medicine, Qiqihar Medical University, Qiqihar 161042, China" |
| ISSN/ISBN: | 1945-239X (Electronic) 0021-9665 (Linking) |
| Abstract: | "Rauvolfia verticillata (Lour.) Baill. (also called Luofumu in Chinese) is commonly used in traditional Chinese medicine for lowering blood pressure. In this study, a high-performance liquid chromatography assay using ultraviolet detection is described for the simultaneous measurement of the five bioactive indole alkaloids (sarpagine, yohimbine, ajmaline, ajmalicine and reserpine) in Rauvolfia. The detection of all five compounds was conducted at 280 nm. In quantitative analysis, the five compounds showed good regressions (R(2) > 0.9988) within the test ranges, and the recovery of the method was in the range of 90.4-101.4%. In addition, a simple gas chromatography mass method using a DB-1 silica capillary column (30 m x 0.25 mm i.d., 0.25 microm) is described for the identification of the highly volatile compounds in Rauvolfia. In qualitative analysis, more than 39 compounds were assayed and identified using the mass function and the National Institute of Standards and Technology database search system. The results demonstrated that the combination of quantitative and qualitative analyses offered an efficient way to evaluate the quality and consistency of Rauvolfia verticillata" |
| Keywords: | "Chromatography, High Pressure Liquid/*methods Gas Chromatography-Mass Spectrometry/*methods Indole Alkaloids/*analysis Limit of Detection Linear Models Rauwolfia/*chemistry Reproducibility of Results Volatile Organic Compounds/*analysis;" |
| Notes: | "MedlineHong, Bo Li, Wenjing Song, Aihua Zhao, Chunjie eng 2012/12/06 J Chromatogr Sci. 2013 Nov-Dec; 51(10):926-30. doi: 10.1093/chromsci/bms191. Epub 2012 Dec 4" |
