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Bull Entomol Res

Title:		Bioactive fractions containing methyl eugenol-derived sex pheromonal components in haemolymph of the male fruit fly Bactrocera dorsalis (Diptera: Tephritidae)
Author(s):		Hee AK; Tan KH;
Address:		"School of Biological Sciences, Universiti Sains Malaysia, Penang, Malaysia. ahee@hortresearch.co.nz"
Journal Title:		Bull Entomol Res
Year:		2005
Volume:		95
Issue:		6
Page Number:		615 - 620
DOI:		10.1079/ber2005392
ISSN/ISBN:		0007-4853 (Print) 0007-4853 (Linking)
Abstract:		"Sex pheromonal components of the tephritid fruit fly Bactrocera dorsalis (Hendel), 2-allyl-4,5-dimethoxyphenol and (E)-coniferyl alcohol, are biosynthesized from a highly potent male attractant, methyl eugenol, then sequestered and stored in the rectal gland prior to their release during courtship at dusk. These sex pheromonal components have been detected in the haemolymph and crop organ. Hence, attempts were made to separate and identify the haemolymph fractions which contained the sex pheromonal components. Identification of these bioactive fractions in methyl eugenol-fed male flies using gel filtration column chromatography and biodetection using live male flies showed two fractions as highly attractive to conspecific males. These fractions show a significant increase in protein absorbance in the elution profile of haemolymph from methyl eugenol-fed males compared with that from methyl eugenol-deprived males. The molecular mass of these bioactive fractions as determined by using gel filtration was in the peptide range of 3.3 to 5.5 kDa. Subsequent gas chromatography-mass spectrometry analyses further confirmed the presence of the pheromonal components in the bioactive fractions. The presence of these methyl eugenol-derived sex pheromonal components in specific haemolymph fractions suggests the involvement of a sex pheromone binding complex"
Keywords:		"Animals Chromatography, Gas Chromatography, Gel Eugenol/*analogs & derivatives/blood Feeding Behavior Hemolymph/chemistry Male Phenols/*blood Sex Attractants/blood/*chemistry Tephritidae/*chemistry;"
Notes:		"MedlineHee, A K W Tan, K H eng Research Support, Non-U.S. Gov't England 2005/12/13 Bull Entomol Res. 2005 Dec; 95(6):615-20. doi: 10.1079/ber2005392"