| Title: | "Functional characterization of an antennal esterase from the noctuid moth, Spodoptera exigua" |
| Author(s): | He P; Zhang J; Li ZQ; Zhang YN; Yang K; Dong SL; He P; |
| Address: | "Education Ministry Key Laboratory of Integrated Management of Crop Diseases and Pests, College of Plant Protection, Nanjing Agricultural University, Nanjing, China" |
| Journal Title: | Arch Insect Biochem Physiol |
| ISSN/ISBN: | 1520-6327 (Electronic) 0739-4462 (Linking) |
| Abstract: | "Odorant-degrading esterases (ODEs) act in the fast deactivation of ester pheromone components and plant volatiles in insects. However, only few ODEs have been characterised to date. In this study, six full-length putative ODE genes (designated SexiCXE4, 5, 17, 18, 20, and 31) were cloned from the male antennae of Spodoptera exigua. The deduced amino acid sequences possessed typical characteristics of a carboxylesterase (CXE) and shared high identities with reported insect CXEs. The tissue and temporal expression patterns were investigated by quantitative real time PCR. Although all six SexiCXEs are expressed in antennae of both sexes, SexiCXE4, 17 and 20 are antennae-enriched; while SexiCXE5 and SexiCXE18 are dominantly expressed in wings, and SexiCXE31 is mainly expressed in proboscises, heads and legs. With the highly biased expression in antennae and proboscises, SexiCXE4 was selected for further functional assay. The recombinant SexiCXE4 were expressed in High-five cells and purified by a Ni(2+) affinity column. SexiCXE4 has much higher enzyme activity against plant volatiles (Z)-3-hexenyl acetate and hexyl acetate than to the sex pheromone components, suggesting that it may function mostly in the degradation of the plant volatiles" |
| Keywords: | Amino Acid Sequence Animals Arthropod Antennae/enzymology Carboxylesterase/chemistry/genetics/*metabolism Insect Proteins/chemistry/genetics/*metabolism Male Molecular Sequence Data Moths/*enzymology/genetics Phylogeny Real-Time Polymerase Chain Reaction; |
| Notes: | "MedlineHe, Peng Zhang, Jin Li, Zhao-Qun Zhang, Ya-Nan Yang, Ke Dong, Shuang-Lin eng Research Support, Non-U.S. Gov't 2014/04/23 Arch Insect Biochem Physiol. 2014 Jun; 86(2):85-99. doi: 10.1002/arch.21164. Epub 2014 Apr 17" |
