« Previous AbstractAnalysing small insect glands with UV-LDI MS: high-resolution spatial analysis reveals the chemical composition and use of the osmeterium secretion in Themira superba (Sepsidae: Diptera)    Next Abstract"In vitro biosynthesis of novel 5beta-reduced steroids by the testis of the round goby, Neogobius melanostomus" »

Trop Biomed

Title:		Bacteria as a source of oviposition attractant for Aedes aegypti mosquitoes
Author(s):		Arbaoui AA; Chua TH;
Address:		"School of Medicine, Universiti Malaysia Sabah, Jalan UMS, 88400 Kota Kinabalu, Sabah, Malaysia"
Journal Title:		Trop Biomed
Year:		2014
Volume:		31
Issue:		1
Page Number:		134 - 142
DOI:
ISSN/ISBN:		2521-9855 (Electronic) 0127-5720 (Linking)
Abstract:		"Since a safe and effective mass vaccination program against dengue fever is not presently available, a good way to prevent and control dengue outbreaks depends mainly on controlling the mosquito vectors. Aedes aegypti mosquito populations can be monitored and reduced by using ovitraps baited with organic infusions. A series of laboratory experiments were conducted which demonstrated that the bacteria in bamboo leaf infusion produce volatile attractants and contact chemical stimulants attractive to the female mosquitoes. The results showed that the female mosquitoes laid most of their eggs (59.9 +/- 8.1 vs 2.9 +/- 2.8 eggs, P<0.001) in bamboo leaf infusions when compared to distilled water. When the fresh infusion was filtered with a 0.45 mum filter membrane, the female mosquitoes laid significantly more eggs (64.1 +/- 6.6 vs 4.9 +/- 2.6 eggs, P<0.001) in unfiltered infusion. However when a 0.8 mum filter membrane was used, the female laid significantly more eggs (62.0 +/- 4.3 vs 10.1 +/- 7.8 eggs, P<0.001) in filtrate compared to a solution containing the residue. We also found that a mixture of bacteria isolated from bamboo leaf infusion serve as potent oviposition stimulants for gravid Aedes mosquitoes. Aedes aegypti laid significantly more eggs (63.3 +/- 6.5 vs 3.1 +/- 2.4 eggs, P<0.001) in bacteria suspension compared to sterile R2A medium. Our results suggest microbial activity has a role in the production of odorants that mediate the oviposition response of gravid mosquitoes"
Keywords:		Aedes/*physiology Animals Bacteria/*chemistry Bambusa/*chemistry/microbiology Dengue/prevention & control Female Insect Vectors/*physiology Oviposition Ovum Plant Leaves/chemistry/microbiology Plant Preparations/*pharmacology Volatile Organic Compounds/*p;
Notes:		"MedlineArbaoui, A A Chua, T H eng Research Support, Non-U.S. Gov't Malaysia 2014/05/28 Trop Biomed. 2014 Mar; 31(1):134-42"