| Title: | "Characterization of Z/E11- and Z9-desaturases from the obliquebanded leafroller moth, Choristoneura rosaceana" |
| Author(s): | Hao G; O'Connor M; Liu W; Roelofs WL; |
| Address: | "Department of Entomology, New York State Agricultural Experiment Station, Cornell University, Geneva, NY 14456, USA" |
| ISSN/ISBN: | 1536-2442 (Electronic) 1536-2442 (Linking) |
| Abstract: | "A (triangle up) 11-desaturase gene was cloned from the sex pheromone gland of the obliquebanded leafroller moth, Choristoneura rosaceana. The desaturase cDNA sequence spans 1300 nucleotides with an open reading frame encoding a 335 amino-acid protein, which has 81% identity to a Z/E11-desaturase of the redbanded leafroller moth, Argyrotaenia velutinana. A functional assay with a pYES2 yeast expression system demonstrated that the (triangle up) 11-desaturase exhibits unusual substrate and stereospecificities in producing a Z/E11 mixture (7:1) of only C14 acids. A metabolic Z9-desaturase also was cloned from fat body of this species, and proved to be in the class that produces more Z9-16:Acid than Z9-18:Acid" |
| Keywords: | "Amino Acid Sequence Animals Cloning, Molecular/methods DNA Primers/chemistry DNA, Complementary/chemistry Fatty Acid Desaturases/chemistry/*genetics/*metabolism Female Gas Chromatography-Mass Spectrometry/methods/veterinary *Genes, Insect Insect Proteins/;" |
| Notes: | "MedlineHao, Guixia O'Connor, Marion Liu, Weitian Roelofs, Wendell L eng Research Support, U.S. Gov't, Non-P.H.S. 2004/09/30 J Insect Sci. 2002; 2:26. doi: 10.1093/jis/2.1.26. Epub 2002 Dec 17" |
