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Insect Mol Biol

Title:		Functional impact of silencing the Helicoverpa armigera sex-peptide receptor on female reproductive behaviour
Author(s):		Hanin O; Azrielli A; Applebaum SW; Rafaeli A;
Address:		"Department of Entomology, Hebrew University, Rehovot, Israel"
Journal Title:		Insect Mol Biol
Year:		2012
Volume:		20111216
Issue:		2
Page Number:		161 - 167
DOI:		10.1111/j.1365-2583.2011.01122.x
ISSN/ISBN:		1365-2583 (Electronic) 0962-1075 (Linking)
Abstract:		"Female Helicoverpa armigera sex pheromone production is under the control of pheromone biosynthesis-activating neuropeptide (PBAN). After mating, females undergo suppression of sex pheromone production and enhanced oviposition as a result of the transfer of male-derived seminal peptides. In a previous study we identified a putative H. armigera sex-peptide receptor (HeaSP-R) and demonstrated a significant up-regulation in gene expression levels of this receptor in brains and pheromone glands of mated females, thereby implicating a regulatory role for sex peptide in the reproductive behaviour of H. armigera. In the present study, we show that virgin females injected with Drosophila melanogaster SP (DrmSP), in addition to inhibition of pheromone production, also exhibited a suppression of calling behaviour and a significant reduction in the gene expression levels of the PBAN-receptor. In addition, RNA interference (RNAi) silencing of the HeaSP-R expression by 50-60% prevented DrmSP-suppression of pheromone production and calling behaviour. Moreover, mated, silenced females failed to increase their oviposition rates as is normally observed in mated females, and their behaviour did not differ from that of virgin females. However, sex pheromone production by mated, silenced females remained low, comparable to mated, normal females, thereby indicating the probable involvement of additional factors in the suppression of sex pheromone production after mating"
Keywords:		"Animals *Drosophila Proteins Female Gene Knockdown Techniques Insect Proteins/genetics/*metabolism Male Moths/genetics/*metabolism Oviposition Ovum/growth & development *Peptides RNA Interference Receptors, Peptide Sex Attractants/metabolism *Sexual Behav;"
Notes:		"MedlineHanin, O Azrielli, A Applebaum, S W Rafaeli, Ada eng Research Support, Non-U.S. Gov't England 2011/12/17 Insect Mol Biol. 2012 Apr; 21(2):161-7. doi: 10.1111/j.1365-2583.2011.01122.x. Epub 2011 Dec 16"