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Nucleic Acids Res

Title:		Defective nucleotide excision repair in yeast hpr1 and tho2 mutants
Author(s):		Gonzalez-Barrera S; Prado F; Verhage R; Brouwer J; Aguilera A;
Address:		"Departamento de Genetica, Facultad de Biologia, Universidad de Sevilla, Avd. Reina Mercedes 6, 41012 Sevilla, Spain"
Journal Title:		Nucleic Acids Res
Year:		2002
Volume:		30
Issue:		10
Page Number:		2193 - 2201
DOI:		10.1093/nar/30.10.2193
ISSN/ISBN:		1362-4962 (Electronic) 0305-1048 (Print) 0305-1048 (Linking)
Abstract:		"Nucleotide excision repair (NER) and transcription are intimately related. First, TFIIH has a dual role in transcription initiation and NER and, secondly, transcription leads to more efficient repair of damage present in transcribed sequences. It is thought that elongating RNAPII, stalled at a DNA lesion, is used for the loading of the NER machinery in a process termed transcription-coupled repair (TCR). Non-transcribed regions are repaired by the so-called global genome repair (GGR). We have previously defined a number of yeast genes, whose deletions confer transcription-dependent hyper-recombination phenotypes. As these mutations cause impairment of transcription elongation we have assayed whether they also affect DNA repair. We show that null mutations of the HPR1 and THO2 genes, encoding two prominent proteins of the THO complex, increase UV sensitivity of yeast cells lacking GGR. Consistent with this result, molecular analyses of DNA repair of the RPB2 transcribed strand using T4 endo V show that hpr1 and tho2 do indeed impair TCR. However, this effect is not confined to TCR alone because the mutants are slightly affected in GGR. These results indicate that THO affects both transcription and NER. We discuss different alternatives to explain the effect of the THO complex on DNA repair"
Keywords:		"Blotting, Northern *Cell Cycle Proteins DNA Repair/*genetics DNA, Fungal/genetics/metabolism/radiation effects *DNA-Binding Proteins Deoxyribonuclease (Pyrimidine Dimer) Dose-Response Relationship, Radiation Endodeoxyribonucleases/metabolism Fungal Protei;"
Notes:		"MedlineGonzalez-Barrera, Sergio Prado, Felix Verhage, Richard Brouwer, Jaap Aguilera, Andres eng Research Support, Non-U.S. Gov't England 2002/05/10 Nucleic Acids Res. 2002 May 15; 30(10):2193-201. doi: 10.1093/nar/30.10.2193"