| Title: | Identification of compounds from non-polar fractions of Blechnum spp and a multitarget approach involving enzymatic modulation and oxidative stress |
| Author(s): | Andrade JM; Maurmann N; Pranke P; Turatti IC; Lopes NP; Henriques AT; |
| Address: | "Laboratory of Pharmacognosy, Faculty of Pharmacy, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, RS, Brazil. Hematology and Stem Cell Laboratory, Faculty of Pharmacy, UFRGS, Porto Alegre, RS, Brazil. Stem Cell Research Institute, Porto Alegre, RS, Brazil. Faculty of Pharmaceutical Sciences of Ribeirao Preto, Universidade de Sao Paulo (USP), Ribeirao Preto, SP, Brazil" |
| ISSN/ISBN: | 2042-7158 (Electronic) 0022-3573 (Linking) |
| Abstract: | "OBJECTIVES: The hexane (HEX) and dichloromethane (DCM) fractions from Blechnum binervatum, Blechnum brasiliense and Blechnum occidentale were studied about phytochemicals and biological properties using multitarget approach. METHODS: The chemical composition was performed by gas chromatography coupled with mass spectrometry detector (GC-MS) analysis. Antioxidant capacity was evaluated against free radicals and on lipid peroxidation. Monoamine oxidases (MAO) and cholinesterases enzymatic modulation, as well as effects on rat and human cells, were assessed. KEY FINDINGS: The CG-MS analysis allowed the identification of a non-polar compound series, being neophytadiene the major constituent in all DCM fractions and in HEX fractions from B. binervatum and B. occidentale. In B. brasiliense HEX fraction, beta-sitosterol was the main compound. In general, B. brasiliense DCM fraction presented the highest antioxidant activity, with IC(50) values around 9, 2 and 1.2 times lower than those found for the other species, against HO , NO and on lipid peroxidation, respectively. Regarding enzyme modulations, B. brasiliense DCM fraction presented higher MAO-A inhibition (IC(50) : 31.83 mug/ml), with a better selectivity index (SI MAO-A/MAO-B: 6.77). The lack of harmful effects was observed in rat cells, also highlighted in the stem cells for all Blechnum samples. CONCLUSION: These findings encourage the search for multibinding natural products, mainly from B. brasiliense DCM fraction" |
| Keywords: | Animals Antioxidants/analysis/*pharmacology Ferns/*chemistry Gas Chromatography-Mass Spectrometry Humans Lipid Peroxidation/drug effects Male Monoamine Oxidase/metabolism Monoamine Oxidase Inhibitors/analysis/*pharmacology Oxidative Stress/*drug effects P; |
| Notes: | "MedlineAndrade, Juliana Maria de Mello Maurmann, Natasha Pranke, Patricia Turatti, Izabel Cristina Casanova Lopes, Norberto Peporine Henriques, Amelia T eng England 2016/10/18 J Pharm Pharmacol. 2017 Jan; 69(1):89-98. doi: 10.1111/jphp.12653. Epub 2016 Oct 17" |
