| Title: | Honing in on phenotypes: comprehensive two-dimensional gas chromatography of herbivory-induced volatile emissions and novel opportunities for system-level analyses |
| Address: | "Department of Molecular Ecology , Max-Planck-Institute for Chemical Ecology , Hans-Knoll-Str. 8, Jena 07745 , Germany" |
| ISSN/ISBN: | 2041-2851 (Print) 2041-2851 (Electronic) |
| Abstract: | "Plant volatile organic compound (VOC) production requires a complex network of biochemical pathways, which, although well mapped from a biochemical point of view, remains only partly understood with regard to its physiological and genetic regulation. Additionally, although analytical procedures for plant VOC measurement have become increasingly faster and more sensitive in recent years, pinpointing relevant shifts in VOC production from the thousands of molecular fragments that are generated by modern mass spectrometer instruments remains challenging. Here we discuss novel opportunities for system-wide analysis provided by the implementation of non-targeted data processing and multivariate statistics in VOC analysis. We illustrate the value of implementing non-targeted data processing with examples of recent findings from our group on the interactive control exerted by salivary components of a lepidopteran herbivore, Manduca sexta, on herbivory-induced VOC emissions in the wild tobacco Nicotiana attenuata. Finally, we briefly discuss the use of multi-platform data integration for probing the nature of metabolic and regulatory systems underlying VOC emissions" |
| Keywords: | Herbivory Nicotiana attenuata metabolomics plant volatiles two-dimensional gas chromatography untargeted analysis.; |
| Notes: | "PubMed-not-MEDLINEGaquerel, Emmanuel Baldwin, Ian T eng England 2013/02/28 AoB Plants. 2013; 5:plt002. doi: 10.1093/aobpla/plt002. Epub 2013 Feb 23" |
