| Title: | Evaluation of in vitro and in vivo safety profile of the Indian traditional medicinal plant Grewia tiliaefolia |
| Author(s): | Dicson SM; Samuthirapandi M; Govindaraju A; Kasi PD; |
| Address: | "Department of Biotechnology, Alagappa University, Karaikudi 630 004, Tamil Nadu, India. Centre for Pheromone Technology, Department of Animal Science, Bharathidasan University, Tiruchirappalli 620 024, Tamil Nadu, India. Department of Biotechnology, Alagappa University, Karaikudi 630 004, Tamil Nadu, India. Electronic address: devikasi@yahoo.com" |
| DOI: | 10.1016/j.yrtph.2015.07.011 |
| ISSN/ISBN: | 1096-0295 (Electronic) 0273-2300 (Linking) |
| Abstract: | "Grewia tiliaefolia Vahl., is a subtropical tree used in Ayurvedic formulations and also by tribal communities in India for treatment of wounds, urinary infection and skin diseases. Despite of its medicinal use, the plant has not been previously tested for its safety. This study evaluated the toxicological profile of the methanolic extract of G. tiliaefolia leaves (MEGT) through in vitro (cytotoxic, mutagenic, genotoxic) and in vivo (acute and sub-acute) studies. In vitro assays conclude that there is no significant (p<0.05) level of cytotoxicity and genotoxicity at the tested concentrations (250-2000 mug/ml) in peripheral blood mononuclear cells. Ames assay in the tested Salmonella typhimurium strains revealed no significant (p<0.05) increase in the mutagenic index both in the presence and absence of metabolic activation. Alternatively, no change in mortality, physiological, behavioural, haematological and biochemical parameters were observed under in vivo acute (250, 500, 1000, 2000 mg/kg b.w) and sub-acute (100, 200, 1000 mg/kg b.w) toxicity studies in Wistar rats. Histopathological examinations of the animals did not reveal any treatment related changes and showed normal architecture, even at the highest concentration (1000 mg/kg b.w) following 28 days treatment. Overall, the results show that MEGT is non-toxic at the tested concentrations and is safe to be used as herbal medicine" |
| Keywords: | "Animals Dose-Response Relationship, Drug Female Grewia/*adverse effects India Leukocytes, Mononuclear/drug effects Male Mutagenicity Tests/methods Plant Extracts/adverse effects Plants, Medicinal/*adverse effects Rats Rats, Wistar Salmonella typhimurium/d;" |
| Notes: | "MedlineDicson, Sheeja Malar Samuthirapandi, Muniasamy Govindaraju, Archunan Kasi, Pandima Devi eng Research Support, Non-U.S. Gov't Netherlands 2015/07/19 Regul Toxicol Pharmacol. 2015 Oct; 73(1):241-7. doi: 10.1016/j.yrtph.2015.07.011. Epub 2015 Jul 15" |
