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Elife

Title:		Genetic architecture of natural variation in cuticular hydrocarbon composition in Drosophila melanogaster
Author(s):		Dembeck LM; Boroczky K; Huang W; Schal C; Anholt RR; Mackay TF;
Address:		"Department of Biological Sciences, North Carolina State University, Raleigh, United States. Genetics Program, North Carolina State University, Raleigh, United States. W. M. Keck Center for Behavioral Biology, North Carolina State University, Raleigh, United States. Department of Entomology, North Carolina State University, Raleigh, United States"
Journal Title:		Elife
Year:		2015
Volume:		20151114
Issue:
Page Number:		-
DOI:		10.7554/eLife.09861
ISSN/ISBN:		2050-084X (Electronic) 2050-084X (Linking)
Abstract:		"Insect cuticular hydrocarbons (CHCs) prevent desiccation and serve as chemical signals that mediate social interactions. Drosophila melanogaster CHCs have been studied extensively, but the genetic basis for individual variation in CHC composition is largely unknown. We quantified variation in CHC profiles in the D. melanogaster Genetic Reference Panel (DGRP) and identified novel CHCs. We used principal component (PC) analysis to extract PCs that explain the majority of CHC variation and identified polymorphisms in or near 305 and 173 genes in females and males, respectively, associated with variation in these PCs. In addition, 17 DGRP lines contain the functional Desat2 allele characteristic of African and Caribbean D. melanogaster females (more 5,9-C27:2 and less 7,11-C27:2, female sex pheromone isomers). Disruption of expression of 24 candidate genes affected CHC composition in at least one sex. These genes are associated with fatty acid metabolism and represent mechanistic targets for individual variation in CHC composition"
Keywords:		Africa Animals Caribbean Region Drosophila melanogaster/*chemistry/*genetics Female Gene Knockout Techniques Hydrocarbons/analysis/*metabolism Integumentary System Male Metabolic Networks and Pathways/*genetics African Drosophila Drosophila genetic refere;
Notes:		"MedlineDembeck, Lauren M Boroczky, Katalin Huang, Wen Schal, Coby Anholt, Robert R H Mackay, Trudy F C eng R01 GM059469/GM/NIGMS NIH HHS/ R01 GM45146/GM/NIGMS NIH HHS/ R01 GM59469/GM/NIGMS NIH HHS/ Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't England 2015/11/17 Elife. 2015 Nov 14; 4:e09861. doi: 10.7554/eLife.09861"