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Comp Biochem Physiol Part D Genomics Proteomics

Title:		Identification and characterization of chemosensory genes in the antennal transcriptome of Spodoptera exigua
Author(s):		Du LX; Liu Y; Zhang J; Gao XW; Wang B; Wang GR;
Address:		"State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, 100193 Beijing, China. Department of Entomology, China Agricultural University, Beijing, China. State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, 100193 Beijing, China. Electronic address: bwang@ippcaas.cn. State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, 100193 Beijing, China. Electronic address: wangguirong@caas.cn"
Journal Title:		Comp Biochem Physiol Part D Genomics Proteomics
Year:		2018
Volume:		20180507
Issue:
Page Number:		54 - 65
DOI:		10.1016/j.cbd.2018.05.001
ISSN/ISBN:		1878-0407 (Electronic) 1744-117X (Linking)
Abstract:		"Chemical senses are crucial for insect behaviors such as host preference, mate choice and oviposition site selection. Various protein families are involved in these processes, including odorant receptors (ORs), ionotropic receptors (IRs), gustatory receptors (GRs), chemosensory proteins (CSPs), odorant binding proteins (OBPs) and sensory neuron membrane proteins (SNMPs). To better understand the olfactory mechanism in Spodoptera exigua, we conducted transcriptome analysis of adult antennae and identified a total of 157 candidate chemosensory genes encoding 51 ORs, 20 IRs, 7 GRs, 32 CSPs, 45 OBPs and 2 SNMPs. Quantitative real time PCR (qPCR) analysis of the tissue- and sex-specific expression profiles of ORs, GRs and IRs revealed that these genes could be detected in at least one tissue tested. SexiOR6, 11, 13 and 16, which were predicted to be pheromone receptors based on phylogenetic analysis, exhibited male-specific antennae expression. SexiOR18, 26, 28, 30, 34, 39, and 40 exhibited female-biased expression. SexiGR1, SexiGR2 and SexiGR3, are predicted carbon dioxide receptors, and the former was expressed specifically in antennae, and the latter two were expressed both in antennae and labial palps. SexiIRs had diverse expression profiles. SexiIR8a and SexiIR25a were quite conserved and expressed at high levels. This work will greatly facilitate the understanding of olfactory system in S. exigua and provides valuable information for further functional studies of the chemoreception mechanism in Lepidopteran moths"
Keywords:		"Animals Arthropod Antennae/*metabolism Female Gene Expression Profiling Insect Proteins/*genetics/metabolism Male Phylogeny Receptors, Cell Surface/*genetics/metabolism Receptors, Odorant/*genetics/metabolism Receptors, Pheromone/*genetics/metabolism Smel;"
Notes:		"MedlineDu, Li-Xiao Liu, Yang Zhang, Jin Gao, Xi-Wu Wang, Bing Wang, Gui-Rong eng Research Support, Non-U.S. Gov't Netherlands 2018/05/23 Comp Biochem Physiol Part D Genomics Proteomics. 2018 Sep; 27:54-65. doi: 10.1016/j.cbd.2018.05.001. Epub 2018 May 7"