| Title: | Influence of Leishmania (Viannia) braziliensis infection on the attractiveness of BALB/c mice to Nyssomyia neivai (Diptera: Psychodidae) |
| Author(s): | da Rocha Silva FB; Miguel DC; Machado VE; Oliveira WHC; Goulart TM; Tosta CD; Pinheiro HP; Pinto MC; |
| Address: | "Departamento de Ciencias Biologicas, Universidade Estadual Paulista (UNESP), Faculdade de Ciencias Farmaceuticas, Campus Araraquara, Araraquara, Sao Paulo, Brasil. Departamento de Biologia Animal, Universidade Estadual de Campinas (UNICAMP), Campinas, Sao Paulo, Brasil. Instituto Federal de Educacao, Ciencia e Tecnologia de Sao Paulo (IFSP), Campus Matao, Matao, Sao Paulo, Brasil. Departamento de Estatistica, Universidade Estadual de Campinas (UNICAMP), Campinas, Sao Paulo, Brasil" |
| DOI: | 10.1371/journal.pone.0214574 |
| ISSN/ISBN: | 1932-6203 (Electronic) 1932-6203 (Linking) |
| Abstract: | "BACKGROUND: Phlebotomine sand flies are vectors for several pathogens, with Leishmania being the most important. In Brazil, the main aetiological agent of American cutaneous leishmaniasis (ACL) is Leishmania (Viannia) braziliensis, and Nyssomyia neivai is one of its main vectors in Sao Paulo state and other areas of South America. Similar to other haematophagous insects, sand flies use volatile compounds called kairomones to locate their hosts for blood meals. A possible increase in the attractiveness of hosts infected with Leishmania infantum to their vectors has been demonstrated. In the present study, we aimed to investigate whether L. braziliensis-infected hosts present higher attractiveness to Ny. neivai and to identify differences in the volatile compounds released by infected and uninfected mice. RESULTS: Behavioural experiments in which sand fly females directly fed on infected or uninfected mice showed no significant differences in the attractiveness of the mice or the blood volume ingested. Y-tube olfactometer bioassays also revealed no significant differences in the attractiveness of these hosts to Ny. neivai. No differences were observed in the profiles of the volatile compounds released by the two groups of mice. However, PCA and cluster analysis were able to classify the 31 identified compounds into three clusters according to their abundances. This classification showed a possible role for individual variation in the absence of differences in volatile profiles and attractiveness between infected and uninfected mice. CONCLUSION: In this first cross-sectional study with an aetiological agent of ACL, there were no statistically significant differences in the attractiveness of infected hosts to their vector" |
| Keywords: | "Animals Behavior, Animal Biological Assay Brazil Cluster Analysis Cross-Sectional Studies *Feeding Behavior Female *Insect Bites and Stings Insect Vectors/*physiology *Leishmania braziliensis Leishmaniasis, Cutaneous/*transmission Male Mice Mice, Inbred B;" |
| Notes: | "Medlineda Rocha Silva, Flavia Benini Miguel, Danilo Ciccone Machado, Vicente Estevam Oliveira, Wanderson Henrique Cruz Goulart, Thais Marchi Tosta, Christiann Davis Pinheiro, Hildete Prisco Pinto, Mara Cristina eng Research Support, Non-U.S. Gov't 2019/04/02 PLoS One. 2019 Apr 1; 14(4):e0214574. doi: 10.1371/journal.pone.0214574. eCollection 2019" |
