« Previous AbstractSilencing of odorant binding protein gene AlinOBP4 by RNAi induces declining electrophysiological responses of Adelphocoris lineolatus to six semiochemicals    Next AbstractWound-induced endogenous jasmonates stunt plant growth by inhibiting mitosis »

Sci China C Life Sci

Title:		Secretory expression of a single-chain insulin precursor in yeast and its conversion into human insulin
Author(s):		Zhang Y; Hu H; Cai R; Feng Y; Zhu S; He Q; Tang Y; Xu M; Xu Y; Zhang X; Liu B; Liang Z;
Address:		"State Key Laboratory of Molecular Biology, Shanghai Institute of Biochemistry, Chinese Academy of Sciences, China"
Journal Title:		Sci China C Life Sci
Year:		1996
Volume:		39
Issue:		3
Page Number:		225 - 233
DOI:
ISSN/ISBN:		1006-9305 (Print) 1006-9305 (Linking)
Abstract:		A synthetic single-chain porcine insulin precursor (PIP) gene and an alpha-mating factor leader sequence (alpha MFL) gene obtained by the PCR method are inserted between the promoter and 3'-terminating sequence of the alcohol dehydrogenase gene ADH1 in plasmid pVT102-U to form plasmid pVT102-U/alpha MFL-PIP. The single-chain insulin precursor is expressed and secreted to the culture medium by Saccharomyces cerevisiae transformed by pVT102-U/alpha MFL-PIP. The precursor is purified and converted into human insulin by tryptic transpeptidation. The purified human insulin is fully active and can be crystallized. The overall yield of human insulin is 25 mg per liter of culture medium
Keywords:		"Base Sequence Cloning, Molecular *Genes, Synthetic Humans Insulin/*biosynthesis/genetics Mating Factor Molecular Sequence Data Peptides/genetics Polymerase Chain Reaction Protein Precursors/*biosynthesis/genetics Saccharomyces cerevisiae/genetics/*metabol;"
Notes:		"MedlineZhang, Y Hu, H Cai, R Feng, Y Zhu, S He, Q Tang, Y Xu, M Xu, Y Zhang, X Liu, B Liang, Z eng Research Support, Non-U.S. Gov't China 1996/06/01 Sci China C Life Sci. 1996 Jun; 39(3):225-33"