| Title: | "Pheromone-binding proteins in the Asian gypsy moth females, Lymantria dispar, recognizing the sex pheromone and plant volatiles" |
| Author(s): | Yu Y; Zhou P; Zhang J; Zheng C; Zhang J; Chen N; |
| Address: | "Institute of Plant Quarantine, Chinese Academy of Inspection and Quarantine, 100176, Beijing, China. Suifenhe Entry-Exit Inspection and Quarantine Bureau, Heilongjiang Province, 157399, Suifenhe, China" |
| Journal Title: | Arch Insect Biochem Physiol |
| ISSN/ISBN: | 1520-6327 (Electronic) 0739-4462 (Linking) |
| Abstract: | "Lepidopterans are known to have different pheromone-binding proteins with differential expression patterns that facilitate specific signal transduction of semiochemicals. Two PBPs of the Asian gypsy moth, Lymantria dispar, were reported to express in both females and males, but their physiological functions were unknown. Results showed that LdisPBP1 and LdisPBP2 were expressed in the sensilla trichodea of males and the s. trichodea and s. basiconica of females. When LdisPBP1 gene was targeted by RNA interference (RNAi) in males, the expression of LdisPBP1 and LdisPBP2 decreased by 69 and 76%, respectively, and when LdisPBP2 gene was targeted by RNAi, they decreased by 60 and 42%, respectively. In females, after treatment with LdisPBP1 dsRNA, LdisPBP1 and LdisPBP2 levels were reduced by 26 and 69%, respectively, and LdisPBP2 dsRNA reduced the relative expression of them by 4 and 62%, respectively. The expression of LdisPBP1 and LdisPBP2 was interdependent. Electroantennogram (EAG) recordings showed that LdisPBPs participate in the recognition of the sex pheromone in males, and the sex pheromone and plant volatiles in females. The function of LdisPBPs represents the sex-specific roles" |
| Keywords: | Animals Carrier Proteins/*metabolism Female *Gene Expression Insect Proteins/*metabolism Male Moths/*metabolism Sensilla/metabolism Sex Attractants/*metabolism Volatile Organic Compounds/*metabolism Electroantennogram (EAG) Lymantria dispar RNA interferen; |
| Notes: | "MedlineYu, Yanxue Zhou, Ping Zhang, Junhua Zheng, Chao Zhang, Jian Chen, Naizhong eng 2018/06/22 Arch Insect Biochem Physiol. 2018 Sep; 99(1):e21477. doi: 10.1002/arch.21477. Epub 2018 Jun 21" |
