| Title: | "Bioprospecting of Coralline Red Alga Amphiroa rigida J.V. Lamouroux: Volatiles, Fatty Acids and Pigments" |
| Author(s): | Cikos AM; Flanjak I; Bojanic K; Babic S; Cizmek L; Coz-Rakovac R; Jokic S; Jerkovic I; |
| Address: | "Department of Process Engineering, Faculty of Food Technology, Josip Juraj Strossmayer University of Osijek, Franje Kuhaca 18, 31000 Osijek, Croatia. Department of Food and Nutrition Research, Faculty of Food Technology, Josip Juraj Strossmayer University of Osijek, Franje Kuhaca 18, 31000 Osijek, Croatia. Laboratory for Biotechnology in Aquaculture, Division of Materials Chemistry, Ruder Boskovic Institute, Bijenicka cesta 54, 10000 Zagreb, Croatia. Department of Organic Chemistry, Faculty of Chemistry and Technology, University of Split, Rudera Boskovica 35, 21000 Split, Croatia" |
| DOI: | 10.3390/molecules26030520 |
| ISSN/ISBN: | 1420-3049 (Electronic) 1420-3049 (Linking) |
| Abstract: | "Due to the lack of phytochemical composition data, the major goals of the present study on Amphiroa rigida J.V. Lamouroux were to: (a) investigate and compare volatilome profiles of fresh and air-dried samples obtained by headspace solid-phase microextraction (HS-SPME) and hydrodistillation (HD) followed by gas chromatography and mass spectrometry (GC/MS) analysis; (b) determine fatty acids profile by gas chromatography with flame ionization detector (GC-FID); (c) obtain the pigment profiles of semipurified extracts by high performance liquid chromatography (HPLC) and (d) evaluate the antioxidant and antimicrobial activities of its less polar fractions. The comparison of headspace of fresh (FrAr) and air-dried (DrAr) samples revealed many similarities regarding the presence and abundance of the major (heptadecane and pentadecane) and minor compounds. The hydrodistillate (HD) of DrAr profile was quite different in comparison to HD-FrAr. The predominant compound in HD-FrAr was (E)-phytol. In HD-DrAr, its percentage was approximately one-half reduced, but the abundance of its degradation product phytone and of unsaturated and oxygenated compounds increased indicating more intense fatty acid decomposition and oxidation during drying. The fatty acid determination revealed that the most dominant was palmitic acid (42.86%) followed by eicosapentaenoic acid (19.14%) and stearic acid (11.65%). Among the pigments, A. rigida contained fucoxanthin (0.63 mg g(-) (1) of dry fraction), lutein (5.83 mg g(-) (1)), beta-carotene (6.18 mg g(-1)) and chlorophyll a (13.65 mg g(-1)). The analyzed less polar fractions of A. rigida exhibited antioxidant scavenging activity with diammonium salt of 2,2'-azino-bis (3-ethylbenzthiazolin-6-yl) sulfonic acid (ABTS) assay up to 3.87 mg g(-1) trolox equivalents (TE), and with the oxygen radical absorbance capacity (ORAC) assay up to 825.63 mumol g(-1) TE (with carotenoids as the major contributors)" |
| Keywords: | "Antioxidants/chemistry Fatty Acids/*chemistry/isolation & purification Gas Chromatography-Mass Spectrometry Phytochemicals/chemistry Pigments, Biological/*chemistry/isolation & purification Rhodophyta/*chemistry Volatile Organic Compounds/*chemistry/isola;" |
| Notes: | "MedlineCikos, Ana-Marija Flanjak, Ivana Bojanic, Krunoslav Babic, Sanja Cizmek, Lara Coz-Rakovac, Rozelindra Jokic, Stela Jerkovic, Igor eng (KK.01.1.1.01)/Croatian Government and the European Union through the European Regional Development Fund - the Competitiveness and Cohesion Operational Programme (KK.01.1.1.01) The Scientific Centre of Excellence for Marine Bioprospecting -BioProCro./ Switzerland 2021/01/28 Molecules. 2021 Jan 20; 26(3):520. doi: 10.3390/molecules26030520" |
