| Title: | "Isolation of a novel gene from Schizosaccharomyces pombe: stm1+ encoding a seven-transmembrane loop protein that may couple with the heterotrimeric Galpha 2 protein, Gpa2" |
| Author(s): | Chung KS; Won M; Lee SB; Jang YJ; Hoe KL; Kim DU; Lee JW; Kim KW; Yoo HS; |
| Address: | "Cell Cycle and Signal Transduction Research Unit, Korea Research Institute of Bioscience and Biotechnology (KRIBB), P. O. Box 115 Yusong, Taejon 305-606, Korea" |
| ISSN/ISBN: | 0021-9258 (Print) 0021-9258 (Linking) |
| Abstract: | "A putative seven transmembrane protein gene, stm1(+), which is required for proper recognition of nitrogen starvation signals, was isolated as a multicopy suppressor of a ras1 synthetic lethal mutant in Schizosaccharomyces pombe. Under nitrogen-deficient conditions, transcription of the stm1 gene was induced; deletion of stm1 was associated with early entry into G(1) arrest. Under nutritionally sufficient conditions, overexpression of Stm1 inhibited vegetative cell growth, resulted in decreased intracellular cAMP levels, increased the expression of the meiosis-specific genes ste11, mei2, and mam2, and facilitated sexual development in homothallic cells. However inhibition of vegetative cell growth and reduction of cAMP levels were not observed in a deletion mutant of the heterotrimeric G protein Galpha2 gene, gpa2, that is responsible for regulating intracellular cAMP levels, a key factor in determining the sexual development in S. pombe. Stm1 protein was shown to interact with Gpa2 through its C-terminal transmembrane domains 5-7. Mutation at Lys(199) in the C-terminal domain (stm1(K199A)) abolished the Stm1 overexpression effect on lowering cAMP levels. Induction of ste11, a meiosis-specific gene transcription factor, by Stm1 overexpression was enhanced in gpa2-deleted cells but was absent in a deletion mutant of sty1, a key protein kinase that links mitotic control with environmental signals and induces stress-responsive genes. Moreover, deletion of both stm1 and ras1 caused delayed entry into G(1) arrest in S. pombe when the cells were grown in a nitrogen-deficient medium. Thus we consider that the stm1 gene can function through Gpa2-dependent and/or -independent pathways and may play a role in providing the prerequisite state for entering the pheromone-dependent differentiation cycle in which heterotrimeric Galpha1 protein, Gpa1, and Ras1 play major roles. Stm1 could function as a sentinel molecule sensing the nutritional state of the cells, stopping the proliferative cell cycle, and preparing the cell to enter meiosis under nutritionally deficient conditions" |
| Keywords: | "Adaptation, Biological Amino Acid Sequence Cell Differentiation Cyclic AMP/metabolism Fungal Proteins/*metabolism *GTP-Binding Protein alpha Subunits GTP-Binding Proteins/genetics/metabolism *Genes, Fungal Heterotrimeric GTP-Binding Proteins/*metabolism M;" |
| Notes: | "MedlineChung, K S Won, M Lee, S B Jang, Y J Hoe, K L Kim, D U Lee, J W Kim, K W Yoo, H S eng Research Support, Non-U.S. Gov't 2001/07/20 J Biol Chem. 2001 Oct 26; 276(43):40190-201. doi: 10.1074/jbc.M100341200. Epub 2001 Jul 18" |
