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Molecules

Title:		Volatile Organic Compounds of Bacillus velezensis GJ-7 against Meloidogyne hapla through Multiple Prevention and Control Modes
Author(s):		Wu W; Zeng Y; Yan X; Wang Z; Guo L; Zhu Y; Wang Y; He X;
Address:		"Key Laboratory of Agricultural Biodiversity and Pest Control, College of Plant Protection, Yunnan Agricultural University, Kunming 650201, China. Key Laboratory of Forest Resources Conservation and Utilization in the Southwest Mountains of China Ministry of Education, Southwest Forestry University, Kunming 650224, China"
Journal Title:		Molecules
Year:		2023
Volume:		20230403
Issue:		7
Page Number:		-
DOI:		10.3390/molecules28073182
ISSN/ISBN:		1420-3049 (Electronic) 1420-3049 (Linking)
Abstract:		"The Bacillus velezensis GJ-7 strain isolated from the rhizosphere soil of Panax notoginseng showed high nematicidal activity and therefore has been considered a biological control agent that could act against the root-knot nematode Meloidogyne hapla. However, little was known about whether the GJ-7 strain could produce volatile organic compounds (VOCs) that were effective in biocontrol against M. hapla. In this study, we evaluated the nematicidal activity of VOCs produced by the fermentation of GJ-7 in three-compartment Petri dishes. The results revealed that the mortality rates of M. hapla J2s were 85% at 24 h and 97.1% at 48 h after treatment with the VOCs produced during GJ-7 fermentation. Subsequently, the VOCs produced by the GJ-7 strain were identified through solid-phase micro-extraction gas chromatography mass spectrometry (SPME-GC/MS). Six characteristic VOCs from the GJ-7 strain fermentation broth were identified, including 3-methyl-1-butanol, 3-methyl-2-pentanone, 5-methyl-2-hexanone, 2-heptanone, 2,5-dimethylpyrazine, and 6-methyl-2-heptanone. The in vitro experimental results from 24-well culture plates showed that the six volatiles had direct-contact nematicidal activity against M. hapla J2s and inhibition activity against egg hatching. In addition, 3-methyl-1-butanol and 2-heptanone showed significant fumigation effects on M. hapla J2s and eggs. Furthermore, all six of the VOCs repelled M. hapla J2 juveniles in 2% water agar Petri plates. The above data suggested that the VOCs of B. velezensis GJ-7 acted against M. hapla through multiple prevention and control modes (including direct-contact nematicidal activity, fumigant activity, and repellent activity), and therefore could be considered as potential biocontrol agents against root-knot nematodes"
Keywords:		Animals *Volatile Organic Compounds/chemistry *Tylenchoidea Antinematodal Agents/pharmacology/chemistry Bacillus velezensis GJ-7 Meloidogyne hapla biological control solid-phase micro-extraction gas chromatography-mass spectrometry volatile organic compou;
Notes:		"MedlineWu, Wentao Zeng, Yuanling Yan, Xirui Wang, Zhuhua Guo, Liwei Zhu, Youyong Wang, Yang He, Xiahong eng CARS-21/China Agriculture Research System/ Switzerland 2023/04/14 Molecules. 2023 Apr 3; 28(7):3182. doi: 10.3390/molecules28073182"