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J Chromatogr B Analyt Technol Biomed Life Sci

Title:		Stability control for breath analysis using GC-MS
Author(s):		Wang XR; Cassells J; Berna AZ;
Address:		"CSIRO Data61, PO Box 76, Epping, NSW 1710, Australia. Electronic address: Rosalind.Wang@csiro.au. CSIRO Health and Biosecurity, GPO Box 1700, Canberra, ACT 2601, Australia. CSIRO Health and Biosecurity, GPO Box 1700, Canberra, ACT 2601, Australia; Department of Pediatrics, Washington University School of Medicine, St. Louis, MO 63110, USA. Electronic address: a.berna@wustl.edu"
Journal Title:		J Chromatogr B Analyt Technol Biomed Life Sci
Year:		2018
Volume:		20180831
Issue:
Page Number:		27 - 34
DOI:		10.1016/j.jchromb.2018.08.024
ISSN/ISBN:		1873-376X (Electronic) 1570-0232 (Print) 1570-0232 (Linking)
Abstract:		"Gas chromatography mass spectrometry (GC-MS) instruments provide researchers and clinicians with a vast amount of information on sample composition, thus these instruments are seen as gold standard in breath analysis research. However, there are many factors that can confound the data measured by GC-MS instruments. These factors will make interpretation of GC-MS data unreliable for breath analysis research. We present in this paper detailed studies of two of these factors: instrument variation over time and chemical degradation of known biomarkers during storage in sorbent tubes. We found that a single quadrupole MS showed larger variability in measurements than a quadrupole time-of-flight MS when the same mixture of chemical standards was analysed for a period of up to 8?ª+weeks. We recommend procedures of normalising the data. Moreover, the stability studies of breath biomarkers like thioethers, previously found indicative of malaria, showed that there is a need to store the samples in sorbent tubes at low temperature, 6?ª+ degrees C, for no more than 20?ª+days to avoid the total decay of the chemicals"
Keywords:		*Breath Tests Gas Chromatography-Mass Spectrometry/instrumentation/methods/*standards Reference Standards Reproducibility of Results Volatile Organic Compounds/*analysis Breath Day of analysis Gas chromatography Mass spectrometry Stability Thioethers Vari;
Notes:		"MedlineWang, X Rosalind Cassells, Julie Berna, Amalia Z eng Netherlands 2018/09/11 J Chromatogr B Analyt Technol Biomed Life Sci. 2018 Oct 15; 1097-1098:27-34. doi: 10.1016/j.jchromb.2018.08.024. Epub 2018 Aug 31"