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Infect Immun

Title:		Tissue-specific adherent Enterococcus faecalis strains that show highly efficient adhesion to human bladder carcinoma T24 cells also adhere to extracellular matrix proteins
Author(s):		Tomita H; Ike Y;
Address:		"Department of Bacteriology and Bacterial Infection Control, Gunma University Graduate School of Medicine, Showa-machi 3-39-22, Maebashi, Gunma 371-8511, Japan. tomitaha@med.gunma-u.ac.jp"
Journal Title:		Infect Immun
Year:		2004
Volume:		72
Issue:		10
Page Number:		5877 - 5885
DOI:		10.1128/IAI.72.10.5877-5885.2004
ISSN/ISBN:		0019-9567 (Print) 1098-5522 (Electronic) 0019-9567 (Linking)
Abstract:		"The ability of Enterococcus faecalis clinical isolates to adhere to immobilized extracellular matrixes (ECMs) coating the walls of microtiter plates was examined by microscopy. The ECMs consisted of fibronectin, laminin, collagen types I, II, IV, and V, fibrinogen, and lactoferrin. With the exception of fibrinogen, each isolate showed a different level of adherence to each of the ECMs. No significant level of adherence to fibrinogen was observed for any isolate. The tissue-specific adhesive strains AS11, AS12, AS14, AS15, HT11, and HT12, which showed highly efficient adherence to human bladder carcinoma T24 cells and human bladder epithelial cells, showed strong adherence to fibronectin, laminin, and collagen type I, II, IV, and V ECMs, and the levels were greater than 10(4) cells/mm2 of well surface coated by ECM. None of the isolates that showed little adherence to human bladder carcinoma T24 cells showed efficient adherence to all the ECMs. The levels of adherence of gelatinase-producing isolates to the collagens were lower than the levels of adherence of gelatinase-negative isolates. When tissue-specific adhesive strains that adhered strongly to each ECM were preincubated with fibronectin, the adherence of the strains to fibronectin was inhibited, but the adherence of the strains to collagen type IV was not inhibited. Likewise, preincubation with collagen type IV inhibited adherence to collagen type IV but not adherence to fibronectin. All of the E. faecalis isolates were shown to carry the ace gene by PCR analysis performed with specific primers for collagen binding domain A of ace. The ace gene encodes Ace (adhesin of collagen from enterococci). The prtF gene of group A streptococci, which encodes the fibronectin binding protein of group A streptococci, was not detected in the tissue-specific adhesive strains by Southern analysis performed with the prtF probe of the Streptococcus pyogenes JRS4 strain. Mutants with altered collagen binding were isolated by insertion of Tn916 into the chromosome of tissue-specific adhesive strain AS14. The number of mutant adhesive bacterial cells that adhered to collagen and also to laminin was 1 or 2 orders lower than the number observed for the wild-type strain, but the level of adherence to fibronectin remained the same as that of the wild-type strain"
Keywords:		"ATP-Binding Cassette Transporters/metabolism Adhesins, Bacterial *Bacterial Adhesion Bacterial Outer Membrane Proteins/metabolism Bacterial Proteins/metabolism Carrier Proteins/metabolism Cell Line, Tumor Collagen/metabolism DNA Transposable Elements/gene;"
Notes:		"MedlineTomita, Haruyoshi Ike, Yasuyoshi eng Research Support, Non-U.S. Gov't 2004/09/24 Infect Immun. 2004 Oct; 72(10):5877-85. doi: 10.1128/IAI.72.10.5877-5885.2004"