« Previous AbstractVolatile organic compound emissions from latex paint--Part 2. Test house studies and indoor air quality (IAQ) modeling    Next AbstractPartial characterization of enterocin MR99 from a corn silage isolate of Enterococcus faecalis »

Insects

Title:		"A Transcriptome Survey Spanning Life Stages and Sexes of the Harlequin Bug, Murgantia histrionica"
Author(s):		Sparks ME; Rhoades JH; Nelson DR; Kuhar D; Lancaster J; Lehner B; Tholl D; Weber DC; Gundersen-Rindal DE;
Address:		"Invasive Insect Biocontrol and Behavior Laboratory, USDA-ARS, Beltsville, MD 20705, USA. michael.sparks@ars.usda.gov. Invasive Insect Biocontrol and Behavior Laboratory, USDA-ARS, Beltsville, MD 20705, USA. joshua.rhoades@ars.usda.gov. Department of Microbiology, Immunology and Biochemistry, University of Tennessee Health Science Center, Memphis, TN 38163, USA. drnelson1@gmail.com. Invasive Insect Biocontrol and Behavior Laboratory, USDA-ARS, Beltsville, MD 20705, USA. daniel.kuhar@ars.usda.gov. Department of Biological Sciences, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061, USA. jlancas7@vt.edu. Department of Biological Sciences, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061, USA. lehnerb@vt.edu. Department of Biological Sciences, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061, USA. tholl@vt.edu. Invasive Insect Biocontrol and Behavior Laboratory, USDA-ARS, Beltsville, MD 20705, USA. don.weber@ars.usda.gov. Invasive Insect Biocontrol and Behavior Laboratory, USDA-ARS, Beltsville, MD 20705, USA. dawn.gundersen-rindal@ars.usda.gov"
Journal Title:		Insects
Year:		2017
Volume:		20170525
Issue:		2
Page Number:		-
DOI:		10.3390/insects8020055
ISSN/ISBN:		2075-4450 (Print) 2075-4450 (Electronic) 2075-4450 (Linking)
Abstract:		"The harlequin bug, Murgantia histrionica (Hahn), is an agricultural pest in the continental United States, particularly in southern states. Reliable gene sequence data are especially useful to the development of species-specific, environmentally friendly molecular biopesticides and effective biolures for this insect. Here, mRNAs were sampled from whole insects at the 2nd and 4th nymphal instars, as well as sexed adults, and sequenced using Illumina RNA-Seq technology. A global assembly of these data identified 72,540 putative unique transcripts bearing high levels of similarity to transcripts identified in other taxa, with over 99% of conserved single-copy orthologs among insects being detected. Gene ontology and protein family analyses were conducted to explore the functional potential of the harlequin bug's gene repertoire, and phylogenetic analyses were conducted on gene families germane to xenobiotic detoxification, including glutathione S-transferases, carboxylesterases and cytochrome P450s. Genic content in harlequin bug was compared with that of the closely related invasive pest, the brown marmorated stink bug, Halyomorpha halys (Stal). Quantitative analyses of harlequin bug gene expression levels, experimentally validated using quantitative real-time PCR, identified genes differentially expressed between life stages and/or sexes"
Keywords:		RNAi targets agricultural pest harlequin bug insect pheromones invasive insects transcriptomics xenobiotic detoxification;
Notes:		"PubMed-not-MEDLINESparks, Michael E Rhoades, Joshua H Nelson, David R Kuhar, Daniel Lancaster, Jason Lehner, Bryan Tholl, Dorothea Weber, Donald C Gundersen-Rindal, Dawn E eng Switzerland 2017/06/08 Insects. 2017 May 25; 8(2):55. doi: 10.3390/insects8020055"