| Title: | "Radiation biology of a serious tropical pigeon pea pest, Maruca vitrata (Fabricius) (Lepidoptera: Crambidae) and potential of radiation mediated 'inherited (F(1)) sterility technique' for the pest suppression" |
| Author(s): | Seth RK; Patil BV; Khan Z; Zarin M; Hanchinal SG; Haveri RV; Gopalkrishna A; |
| Address: | "Department of Zoology, University of Delhi, Delhi, India. Department of Entomology, University of Agricultural Sciences, Raichur, India" |
| DOI: | 10.1080/09553002.2020.1707323 |
| ISSN/ISBN: | 1362-3095 (Electronic) 0955-3002 (Linking) |
| Abstract: | "Purpose: The impact of gamma radiation on the reproductive biology of the spotted pod borer, Maruca vitrata (Fabr.) (Lepidoptera: Crambidae) was ascertained to explore the potential of the radio-genetic 'Inherited (F(1)) Sterility' technique (modified Sterile Insect technique) to control this serious pigeonpea pest in India.Materials and methods: Radio-biological investigations involved dose-response studies, at a range of 100-250 Gy, with respect to radiation induced adverse effects on reproductive behavior in substerilized parent (P(1)) moths, inheritance of sterility and associated metamorphic disruption in F(1) progeny. The reproductive competence of P(1) male moths and their F(1) progeny was evaluated by ascertaining the survival, mating success, sperm transfer and amphimixis of gametes from irradiated male parents and their F(1) progeny. Daily ovipositional and egg fertility profile were evaluated as crucial parameters for simulation modeling for this radio-genetic technique.Results: Radiation-induced check in insemination rate and sterility in P(1) and F(1) generation had a positive correlation with gamma dose. The metamorphic adverse effects in F(1) progeny of substerilized P(1) males were influenced by P(1) irradiation. A higher dose of 200 Gy that resulted in 21.1% fertility in parent cross (P(1)male symbolx Nfemale symbol) could form only 14.4% F(1) adults that were all malformed. A gamma dose of 150 Gy (administered to P(1) males) could be considered as a suitable dose for F(1) sterility, that induced 57.4% control of reproduction in parent generation, followed by 72-85% control of reproduction in F(1) progeny involved in three crosses -F(1)male symbolxNfemale symbol, Nmale symbolxF(1)female symbol, F(1)male symbolxF(1)female symbol. Daily fertility profile of the eggs laid indicated a marked reduction on 4th day onwards during the ovipositional span in P(1) and F(1) generation. High degree of mating competitiveness value (CV) was noticed for 150 Gy irradiated male P(1) moths (0.78-0.93), and their F(1) male progeny (0.70-0.89), released in different sex ratios in the field cages having untreated moths. Reduced relative damage to the pigeon pea plants by the released F(1) progeny in the field cages was observed in comparison to control.Conclusion: The present study indicated the potential use of a dose of 150 Gy to apply the 'F(1) sterility' technique for parabiological suppression of the pigeon pea pod borer. The present findings and reproductive performance of the irradiated moths in F(2) generation along with other compatible biorational pest control tactics (that are in progress), might help in formulating an effective integrated pest management module, including the F(1) sterility technique as an integral component" |
| Keywords: | "Animals Cajanus Female Gamma Rays Infertility/*etiology Male Metamorphosis, Biological/radiation effects Moths/physiology/*radiation effects Pest Control, Biological/*methods Radiation Dosage Radiobiology Sex Ratio F1 sterility Maruca vitrata Sterile Inse;" |
| Notes: | "MedlineSeth, Rakesh K Patil, Basavaraj V Khan, Zubeda Zarin, Mahtab Hanchinal, Shivanand G Haveri, Rachappa V Gopalkrishna, Akshatha eng Research Support, Non-U.S. Gov't England 2019/12/21 Int J Radiat Biol. 2020 Apr; 96(4):532-544. doi: 10.1080/09553002.2020.1707323. Epub 2020 Jan 9" |
