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BMC Plant Biol

Title:		RNA sequencing analysis of Cymbidium goeringii identifies floral scent biosynthesis related genes
Author(s):		Ramya M; Park PH; Chuang YC; Kwon OK; An HR; Park PM; Baek YS; Kang BC; Tsai WC; Chen HH;
Address:		"Floriculture Research Division, National Institute of Horticultural & Herbal Science (NIHHS), Rural Development Administration (RDA), Wanju, 55365, South Korea. Floriculture Research Division, National Institute of Horticultural & Herbal Science (NIHHS), Rural Development Administration (RDA), Wanju, 55365, South Korea. puehee@korea.kr. Department of Life Sciences, National Cheng Kung University (NCKU), Tainan, 701, Taiwan. puehee@korea.kr. Department of Horticultural Science and Biotechnology, Seoul National University (SNU), Seoul, 08826, South Korea. puehee@korea.kr. Department of Life Sciences, National Cheng Kung University (NCKU), Tainan, 701, Taiwan. Department of Horticultural Science and Biotechnology, Seoul National University (SNU), Seoul, 08826, South Korea. Institute of Tropical Plant Sciences, National Cheng Kung University, Tainan, 701, Taiwan. Orchid Research and Development Center, National Cheng Kung University, Tainan, 701, Taiwan. Department of Life Sciences, National Cheng Kung University (NCKU), Tainan, 701, Taiwan. hhchen@mail.ncku.edu.tw. Institute of Tropical Plant Sciences, National Cheng Kung University, Tainan, 701, Taiwan. hhchen@mail.ncku.edu.tw"
Journal Title:		BMC Plant Biol
Year:		2019
Volume:		20190802
Issue:		1
Page Number:		337 -
DOI:		10.1186/s12870-019-1940-6
ISSN/ISBN:		1471-2229 (Electronic) 1471-2229 (Linking)
Abstract:		"BACKGROUND: Cymbidium goeringii belongs to the Orchidaceae, which is one of the most abundant angiosperm families. Cymbidium goeringii consist with high economic value and characteristics include fragrance and multiple flower colors. Floral scent is one of the important strategies for ensuring fertilization. However, limited genetic data is available in this non-model plant, and little known about the molecular mechanism responsible for floral scent in this orchid. Transcriptome and expression profiling data are needed to identify genes and better understand the biological mechanisms of floral scents in this species. Present transcriptomic data provides basic information on the genes and enzymes related to and pathways involved in flower secondary metabolism in this plant. RESULTS: In this study, RNA sequencing analyses were performed to identify changes in gene expression and biological pathways related scent metabolism. Three cDNA libraries were obtained from three developmental floral stages: closed bud, half flowering stage and full flowering stage. Using Illumina technique 159,616,374 clean reads were obtained and were assembled into 85,868 final unigenes (average length 1194 nt), 33.85% of which were annotated in the NCBI non redundant protein database. Among this unigenes 36,082 were assigned to gene ontology and 23,164 were combined with COG groups. Total 33,417 unigenes were assigned in 127 pathways according to the Kyoto Encyclopedia of Genes and Genomes pathway database. According these transcriptomic data we identified number of candidates genes which differentially expressed in different developmental stages of flower related to fragrance biosynthesis. In q-RT-PCR most of the fragrance related genes highly expressed in half flowering stage. CONCLUSIONS: RNA-seq and DEG data provided comprehensive gene expression information at the transcriptional level that could be facilitate the molecular mechanisms of floral biosynthesis pathways in three developmental phase's flowers in Cymbidium goeringii, moreover providing useful information for further analysis on C. goeringii, and other plants of genus Cymbidium"
Keywords:		"Acetates/metabolism Cyclopentanes/metabolism Farnesol/metabolism Flowers/growth & development/*metabolism Gene Expression Profiling Gene Expression Regulation, Plant Genes, Plant/*genetics/physiology *Odorants Orchidaceae/*genetics/metabolism Oxylipins/me;"
Notes:		"MedlineRamya, Mummadireddy Park, Pue Hee Chuang, Yu-Chen Kwon, Oh Keun An, Hye Ryun Park, Pil Man Baek, Yun Su Kang, Byoung-Chorl Tsai, Wen-Chieh Chen, Hong-Hwa eng PJ011832022017/National Institute of Horticulture and Herbal Science/ England 2019/08/04 BMC Plant Biol. 2019 Aug 2; 19(1):337. doi: 10.1186/s12870-019-1940-6"