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Peptides

Title:		Synthesis and biological activity of a photoaffinity-biotinylated pheromone-biosynthesis activating neuropeptide (PBAN) analog
Author(s):		Rafaeli A; Gileadi C;
Address:		"Department of Stored Products, Volcani Center, Bet Dagan, Israel. vtada@volcani.agri.gov.il"
Journal Title:		Peptides
Year:		1999
Volume:		20
Issue:		7
Page Number:		787 - 794
DOI:		10.1016/s0196-9781(99)00063-7
ISSN/ISBN:		0196-9781 (Print) 0196-9781 (Linking)
Abstract:		"To study the mode of action of pheromone-biosynthesis activating neuropeptide (PBAN) at the receptor level and for receptor purification, we synthesized and tested the biologic properties of a photoaffinity biotinylated PBAN analog N-[N-(4-azido-tetrafluorobenzoyl)-biocytinyloxyl-succinimide (Atf-Bct-NHS-PBAN). The Atf-Bct-NHS-PBAN was separated from unreacted reagent and synthetic Hez-PBAN by high-performance liquid chromatography. Conjugated biotin was detected by using enzyme-linked assay as well as tricine sodium dodecyl sulfate polyacrylamide gel electrophoresis. The biologic activity of purified Atf-Bct-NHS-PBAN was confirmed using both in vivo and in vitro pheromonotropic bioassays. These observations indicate that Atf-Bct-NHS-PBAN is a full agonist of PBAN action in pheromone glands and may be used to study PBAN receptors by employing avidin coupled to various reporter groups"
Keywords:		"Affinity Labels/*chemical synthesis Animals Avidin Biological Assay Biotin Female In Vitro Techniques Male Moths Neuropeptides/*chemical synthesis/*pharmacology Pheromones/*biosynthesis Receptors, Neuropeptide/drug effects;"
Notes:		"MedlineRafaeli, A Gileadi, C eng Research Support, Non-U.S. Gov't 1999/09/07 Peptides. 1999; 20(7):787-94. doi: 10.1016/s0196-9781(99)00063-7"