| Title: | Role of Volatiles from the Endophytic Fungus Trichoderma asperelloides PSU-P1 in Biocontrol Potential and in Promoting the Plant Growth of Arabidopsis thaliana |
| Author(s): | Phoka N; Suwannarach N; Lumyong S; Ito SI; Matsui K; Arikit S; Sunpapao A; |
| Address: | "Ratchaburi Campus, King Mongkut's University of Technology Thonburi, Ratchaburi 70150, Thailand. Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. Research Center of Microbial Diversity and Sustainable Utilization, Chiang Mai University, Chiang Mai 50200, Thailand. Department of Biological and Environmental Sciences, Graduate School of Science and Technology for Innovation, Yamaguchi University, Yamaguchi 753-8515, Japan. Research Center for Thermotolerant Microbial Resources (RCTMR), Yamaguchi University, Yamaguchi 753-8515, Japan. Department of Biological Chemistry, Graduate School of Science and Technology for Innovation, Yamaguchi University, Yamaguchi 753-8515, Japan. Department of Agronomy, Faculty of Agriculture at Kamphaeng Saen, Kasetsart University Kamphaeng Saen Campus, Nakhon Pathom 73140, Thailand. Agricultural Innovation and Management Division, Faculty of Natural Resources, Prince of Songkla University, Hatyai, Songkhla 90112, Thailand" |
| ISSN/ISBN: | 2309-608X (Electronic) 2309-608X (Linking) |
| Abstract: | "Fungal volatile organic compounds (VOCs) emitted by Trichoderma species interact with a plant host and display multifaceted mechanisms. In this study, we investigated the antifungal activity of VOCs emitted by Trichoderma asperelloides PSU-P1 against fungal pathogens, as well as the ability of VOCs to activate defense responses and to promote plant growth in Arabidopsis thaliana. The strain's VOCs had remarkable antifungal activity against fungal pathogens, with an inhibition range of 15.92-84.95% in a volatile antifungal bioassay. The VOCs of T. asperelloides PSU-P1 promoted the plant growth of A. thaliana, thereby increasing the fresh weight, root length, and chlorophyll content in the VOC-treated A. thaliana relative to those of the control. High expression levels of the chitinase (CHI) and beta-1,3-glucanase (GLU) genes were found in the VOC-treated A. thaliana by quantitative reverse transcription polymerase chain reaction (RT-PCR). The VOC-treated A. thaliana had higher defense-related enzyme (peroxidase (POD)) and cell wall-degrading enzyme (chitinase and beta-1,3-glucanase) activity than in the control. The headspace VOCs produced by PSU-P1, trapped with solid phase microextraction, and tentatively identified by gas chromatography-mass spectrometry, included 2-methyl-1-butanol, 2-pentylfuran, acetic acid, and 6-pentyl-2H-pyran-2-one (6-PP). The results suggest that T. asperelloides PSU-P1 emits VOCs responsible for antifungal activity, for promoting plant growth, and for inducing defense responses in A. thaliana" |
| Keywords: | Arabidopsis Trichoderma biocontrol agent volatile compounds; |
| Notes: | "PubMed-not-MEDLINEPhoka, Nongnat Suwannarach, Nakarin Lumyong, Saisamorn Ito, Shin-Ichi Matsui, Kenji Arikit, Siwaret Sunpapao, Anurag eng NRCT5-RSA63022-01/National Research Council of Thailand/ -/Prince of Songkla University/ Switzerland 2020/12/10 J Fungi (Basel). 2020 Dec 6; 6(4):341. doi: 10.3390/jof6040341" |
