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In Silico Biol

Title:		"Prediction of 3-dimensional structure of salivary odorant-binding protein-2 of the mosquito Culex quinquefasciatus, the vector of human lymphatic filariasis"
Author(s):		Paramasivan R; Sivaperumal R; Dhananjeyan KJ; Thenmozhi V; Tyagi BK;
Address:		"Centre for Research in Medical Entomology, Madurai, Tamil Nadu, India. rpsivan2000@yahoo.co.in"
Journal Title:		In Silico Biol
Year:		2007
Volume:		7
Issue:		1
Page Number:		1 - 6
DOI:
ISSN/ISBN:		1386-6338 (Print) 1386-6338 (Linking)
Abstract:		"Olfaction of insects is currently recognized as the major area of research for developing novel control strategies to prevent mosquito-borne infections. A 3-dimensional model (3D) was developed for the salivary gland odorant-binding protein-2 of the mosquito Culex quinquefasciatus, a major vector of human lymphatic filariasis. A homology modeling method was used for the prediction of the structure. For the modeling, two template proteins were obtained by mGenTHERADER, namely the high-resolution X-ray crystallography structure of a pheromone-binding protein (ASP1) of Apis mellifera L., [1R5R:A] and the aristolochene synthase from Penicillium roqueforti [1DI1:B]. By comparing the template protein a rough model was constructed for the target protein using MODELLER, a program for comparative modelling. The structure of OBP of the mosquito Culex quinquefasciatus resembles the structure of pheromone-binding protein ASP1 of Apis mellifera L., [1R5R:A]. From Ramachandran plot analysis it was found that the portion of residues falling into the most favoured regions was 86.0%. The predicted 3-D model may be further used in characterizing the protein in wet laboratory"
Keywords:		"Animals Anopheles/metabolism Carrier Proteins/chemistry Computational Biology/methods Crystallography, X-Ray Culex/*metabolism Drosophila melanogaster/metabolism Elephantiasis, Filarial/*transmission Humans Imaging, Three-Dimensional Insect Proteins/chemi;"
Notes:		"MedlineParamasivan, Rajaiah Sivaperumal, Ramamoorthy Dhananjeyan, Kutty Jegadeeswaran Thenmozhi, Velayutham Tyagi, Brij Kishore eng Netherlands 2007/08/11 In Silico Biol. 2007; 7(1):1-6"