| Title: | Polygenic molecular architecture underlying non-sexual cell aggregation in budding yeast |
| Author(s): | Li J; Wang L; Wu X; Fang O; Wang L; Lu C; Yang S; Hu X; Luo Z; |
| Address: | "Laboratory of Population & Quantitative Genetics, State Key Laboratory of Genetic Engineering, School of Life Sciences, Fudan University, Shanghai 200433, China" |
| ISSN/ISBN: | 1756-1663 (Electronic) 1340-2838 (Print) 1340-2838 (Linking) |
| Abstract: | "Cell aggregation in unicellular organisms, induced by either cell non-sexual adhesion to yield flocs and biofilm, or pheromone-driving sexual conjugation is of great significance in cellular stress response, medicine, and brewing industries. Most current literatures have focused on one form of cell aggregation termed flocculation and its major molecular determinants, the flocculation (FLO) family genes. Here, we implemented a map-based approach for dissecting the molecular basis of non-sexual cell aggregation in Saccharomyces cerevisiae. Genome-wide mapping has identified four major quantitative trait loci (QTL) underlying nature variation in the cell aggregation phenotype. High-resolution mapping following up with knockout and allele replacement experiments resolved the QTL into the underlying genes (AMN1, RGA1, FLO1, and FLO8) or even into the causative nucleotide. Genetic variation in the QTL genes can explain up to 46% of phenotypic variation of this trait. Of these genes, AMN1 plays the leading role, differing from the FLO family members, in regulating expression of cell clumping phenotype through inducing cell segregation defect. These findings provide novel insights into the molecular mechanism of how cell aggregation is regulated in budding yeast, and the data will be directly implicated to understand the molecular basis and evolutionary implications of cell aggregation in other fungus species" |
| Keywords: | "Amino Acid Sequence Cell Aggregation Cell Cycle Proteins/genetics Chromosome Mapping Databases, Genetic Flocculation GTPase-Activating Proteins/genetics Gene Knockout Techniques/methods *Genes, Fungal Genetic Variation Genotype Mannose-Binding Lectins/gen;" |
| Notes: | "MedlineLi, Jiarui Wang, Lin Wu, Xiaoping Fang, Ou Wang, Luwen Lu, Chenqi Yang, Shengjie Hu, Xiaohua Luo, Zewei eng Research Support, Non-U.S. Gov't England 2013/01/04 DNA Res. 2013 Feb; 20(1):55-66. doi: 10.1093/dnares/dss033. Epub 2013 Jan 2" |
