| Title: | (S)-Reutericyclin: Susceptibility Testing and In Vivo Effect on Murine Fecal Microbiome and Volatile Organic Compounds |
| Author(s): | Kienesberger B; Obermuller B; Singer G; Mittl B; Grabherr R; Mayrhofer S; Heinl S; Stadlbauer V; Horvath A; Miekisch W; Fuchs P; Klymiuk I; Till H; Castellani C; |
| Address: | "Department of Paediatric and Adolescent Surgery, Medical University of Graz, 8036 Graz, Austria. Department of Biotechnology, University of Natural Resources and Life Sciences Vienna, 1190 Vienna, Austria. Department of Internal Medicine, Division of Gastroenterology and Hepatology, Medical University of Graz, 8036 Graz, Austria. Center of Biomarker Research (CBmed), 8036 Graz, Austria. Experimental Research Center, Department of Anesthesiology and Intensive Care, Rostock University Medical Center, 18057 Rostock, Germany. Gottfried Schatz Research Center, Department of Cell Biology, Histology and Embryology, Medical University of Graz, 8036 Graz, Austria" |
| ISSN/ISBN: | 1422-0067 (Electronic) 1422-0067 (Linking) |
| Abstract: | "We aimed to assess the in vitro antimicrobial activity and the in vivo effect on the murine fecal microbiome and volatile organic compound (VOC) profile of (S)-reutericyclin. The antimicrobial activity of (S)-reutericyclin was tested against Clostridium difficile, Listeria monocytogenes, Escherichia coli, Enterococcus faecium, Staphylococcus aureus, Staphylococcus (S.) epidermidis, Streptococcus agalactiae, Pseudomonas aeruginosa and Propionibacterium acnes. Reutericyclin or water were gavage fed to male BALBc mice for 7 weeks. Thereafter stool samples underwent 16S based microbiome analysis and VOC analysis by gas chromatography mass spectrometry (GC-MS). (S)-reutericyclin inhibited growth of S. epidermidis only. Oral (S)-reutericyclin treatment caused a trend towards reduced alpha diversity. Beta diversity was significantly influenced by reutericyclin. Linear discriminant analysis Effect Size (LEfSe) analysis showed an increase of Streptococcus and Muribaculum as well as a decrease of butyrate producing Ruminoclostridium, Roseburia and Eubacterium in the reutericyclin group. VOC analysis revealed significant increases of pentane and heptane and decreases of 2,3-butanedione and 2-heptanone in reutericyclin animals. The antimicrobial activity of (S)-reutericyclin differs from reports of (R)-reutericyclin with inhibitory effects on a multitude of Gram-positive bacteria reported in the literature. In vivo (S)-reutericyclin treatment led to a microbiome shift towards dysbiosis and distinct alterations of the fecal VOC profile" |
| Keywords: | "Animals Discriminant Analysis Feces/*microbiology Male Mice, Inbred BALB C Microbial Sensitivity Tests Microbiota/*drug effects Tenuazonic Acid/*analogs & derivatives/pharmacology Volatile Organic Compounds/*analysis Voc antibiotic antimicrobial activity;" |
| Notes: | "MedlineKienesberger, Bernhard Obermuller, Beate Singer, Georg Mittl, Barbara Grabherr, Reingard Mayrhofer, Sigrid Heinl, Stefan Stadlbauer, Vanessa Horvath, Angela Miekisch, Wolfram Fuchs, Patricia Klymiuk, Ingeborg Till, Holger Castellani, Christoph eng 867991/Osterreichische Forschungsforderungsgesellschaft/ Switzerland 2021/07/03 Int J Mol Sci. 2021 Jun 15; 22(12):6424. doi: 10.3390/ijms22126424" |
