« Previous Abstract"Sick building syndrome by indoor air pollution in Dalian, China"    Next AbstractStudy on the kinetic characteristics of trace harmful gases for a two-person-30-day integrated CELSS test »

Genomics

Title:		Validation of reference-assisted assembly using existing and novel Heliothine genomes
Author(s):		Guo R; Papanicolaou A; Fritz ML;
Address:		"Department of Entomology, University of Maryland, College Park, MD 20742, USA; Biological Sciences Graduate Program (BISI), Computational Biology, Bioinformatics and Genomics concentration, University of Maryland, College Park, MD 20742, USA. Hawkesbury Institute for the Environment, Western Sydney University, Richmond, NSW 2753, Australia. Electronic address: a.papanicolaou@westernsydey.edu.au. Department of Entomology, University of Maryland, College Park, MD 20742, USA; Biological Sciences Graduate Program (BISI), Computational Biology, Bioinformatics and Genomics concentration, University of Maryland, College Park, MD 20742, USA. Electronic address: mfritz13@umd.edu"
Journal Title:		Genomics
Year:		2022
Volume:		20220803
Issue:		5
Page Number:		110441 -
DOI:		10.1016/j.ygeno.2022.110441
ISSN/ISBN:		1089-8646 (Electronic) 0888-7543 (Linking)
Abstract:		"Chloridea subflexa and Chloridea virescens are a pair of closely related noctuid species exhibiting pheromone-based sexual isolation and divergent host plant preferences. We produced a novel Illumina short read C. subflexa genome assembly and an improved C. virescens genome assembly, which offer opportunities to study the genomic basis for evolutionarily important traits in this lepidopteran family with few genomic resources. We then examined the feasibility of reference-assisted assembly, an approach that leverages existing high quality genomic resources for genome improvement in closely related taxa and applied it to our Heliothine genomes. Our work demonstrates that reference-assisted assembly has the potential to enhance contiguity and completeness of existing insect genomic resources with minimal additional laboratory costs. We conclude by discussing both the potential and pitfalls of reference-assisted assembly according to the intended downstream assembly application"
Keywords:		Animals Genome *Moths/genetics Pheromones Chloridea (Heliothis) subflexa Genome assembly Heliothinae Reference-assisted assembly approach;
Notes:		"MedlineGuo, Rong Papanicolaou, Alexie Fritz, Megan L eng Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. 2022/08/06 Genomics. 2022 Sep; 114(5):110441. doi: 10.1016/j.ygeno.2022.110441. Epub 2022 Aug 3"