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Int J Mol Sci

Title:		The Sequence Characteristics and Binding Properties of the Odorant-Binding Protein 2 of Euplatypus parallelus to Semiochemicals
Author(s):		Cui G; Zhou X; Wang Q; Zhang K; Qin L; Guo J;
Address:		"Key Laboratory of Green Prevention and Control of Tropical Plant Diseases and Pests (Ministry of Education), School of Plant Protection, Hainan University, Haikou 570228, China"
Journal Title:		Int J Mol Sci
Year:		2023
Volume:		20230115
Issue:		2
Page Number:		-
DOI:		10.3390/ijms24021714
ISSN/ISBN:		1422-0067 (Electronic) 1422-0067 (Linking)
Abstract:		"Euplatypus parallelus is one of the dominant rubber bark beetle species in Hainan's rubber-planting area. Semiochemicals, including the volatiles found in rubber trees and aggregation pheromones, play an important role in the search for suitable host plants. To examine the possible functional role of highly expressed odorant-binding protein 2 of Euplatypus parallelus (EparOBP2) in the semiochemical recognition process, we cloned and analyzed the cDNA sequence of EparOBP2. The results showed that EparOBP2 contains an open reading frame (ORF) of 393 bp that encodes 130 amino acids, including a 21-amino-acid residue signal peptide at the N-terminus. The matured EparOBP2 protein consists of seven alpha-helices, creating an open binding pocket and three disulfide bridges. The results of the fluorescence binding assay showed that EparOBP2 had high binding ability with alpha-pinene and myrcene. The docking results confirmed that the interactions of alpha-pinene and myrcene with EparOBP2 were primarily achieved through hydrophobic interactions. This study provides evidence that EparOBP2 may be involved in the chemoreception of semiochemicals and that it can successfully contribute to the integrated management of E. parallelus"
Keywords:		"Animals Pheromones/metabolism Rubber *Weevils/metabolism *Receptors, Odorant/metabolism Insect Proteins/metabolism Protein Binding Euplatypus parallelus binding ability fluorescence competitive binding assays molecular docking odorant-binding proteins;"
Notes:		"MedlineCui, Guangchao Zhou, Xiang Wang, Qian Zhang, Kai Qin, Lei Guo, Jixing eng 2019RC136/Natural Science Foundation of Hainan Province/ 322MS012/Natural Science Foundation of Hainan Province/ 32060644/National Natural Science Foundation of China/ KYQD (ZR)1879/Hainan University/ Switzerland 2023/01/22 Int J Mol Sci. 2023 Jan 15; 24(2):1714. doi: 10.3390/ijms24021714"