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FEBS Lett

Title:		STE2/SCG1-dependent inhibition of STE4-induced growth arrest by mutant STE4 delta C6 in the yeast pheromone response pathway
Author(s):		Coria R; Savinon-Tejeda AL; Birnbaumer L;
Address:		"Departamento de Microbiologia, Universidad Nacional Autonoma de Mexico, Mexico, D.F"
Journal Title:		FEBS Lett
Year:		1995
Volume:		367
Issue:		2
Page Number:		122 - 126
DOI:		10.1016/0014-5793(95)00526-f
ISSN/ISBN:		0014-5793 (Print) 0014-5793 (Linking)
Abstract:		"The yeast pheromone response pathway involves the activation of a heterotrimeric G protein composed by SCG1 (alpha) (also GPA1), STE4 (beta), and STE18 (gamma) subunits by the pheromone-activated receptors STE2 and STE3 in a and alpha cells, respectively. Upon exchange of bound GDP for GTP in the SCG1 subunit, the release of STE4/STE18 dimer occurs which, in turn causes activation of downstream effectors leading growth arrest and mating competence. Over-expression of STE4 also leads to growth arrest in a STE18 dependent manner. Removal of 6 amino acids from the C-terminus of STE4 rendered a subunit incapable of downstream signalling but still able to interact with STE18. This delta C6 mutant acts as a dominant negative because it blocks the growth arresting effect obtained by over-expression of STE4. The inhibitory effect of STE4 delta C6 is dependent on the presence of the SCG1 subunit in a STE2 but not ste2 background. Inhibition of the growth arresting effect of STE4 by the delta C6 mutant is not due to competition at the effector site, but rather involves an intrinsic activity of STE2 that is dependent on SCG1"
Keywords:		"*GTP-Binding Protein alpha Subunits GTP-Binding Protein alpha Subunits, Gq-G11 *GTP-Binding Protein beta Subunits GTP-Binding Proteins/genetics/*physiology *Heterotrimeric GTP-Binding Proteins Mating Factor Mutation Peptides/pharmacology Pheromones/pharma;"
Notes:		"MedlineCoria, R Savinon-Tejeda, A L Birnbaumer, L eng DK-19318/DK/NIDDK NIH HHS/ Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. England 1995/06/26 FEBS Lett. 1995 Jun 26; 367(2):122-6. doi: 10.1016/0014-5793(95)00526-f"