| Title: | "Characterization and expression of the gene encoding En-MAPK1, an intestinal cell kinase (ICK)-like kinase activated by the autocrine pheromone-signaling loop in the Polar Ciliate, Euplotes nobilii" |
| Author(s): | Candelori A; Luporini P; Alimenti C; Vallesi A; |
| Address: | "Laboratory of Eukaryotic Microbiology and Animal Biology, Department of Environmental and Natural Sciences, University of Camerino, Camerino 62032, Italy. adriana.vallesi@unicam.it" |
| ISSN/ISBN: | 1422-0067 (Print) 1422-0067 (Electronic) 1422-0067 (Linking) |
| Abstract: | "In the protozoan ciliate Euplotes, a transduction pathway resulting in a mitogenic cell growth response is activated by autocrine receptor binding of cell type-specific, water-borne signaling protein pheromones. In Euplotes raikovi, a marine species of temperate waters, this transduction pathway was previously shown to involve the phosphorylation of a nuclear protein kinase structurally similar to the intestinal-cell and male germ cell-associated kinases described in mammals. In E. nobilii, which is phylogenetically closely related to E. raikovi but inhabits Antarctic and Arctic waters, we have now characterized a gene encoding a structurally homologous kinase. The expression of this gene requires +1 translational frameshifting and a process of intron splicing for the production of the active protein, designated En-MAPK1, which contains amino acid substitutions of potential significance for cold-adaptation" |
| Keywords: | "Amino Acid Sequence Autocrine Communication/*physiology Cloning, Molecular *Euplotes/enzymology/genetics Gene Expression Regulation, Enzymologic/*physiology Humans MAP Kinase Signaling System/*physiology Male *Mitogen-Activated Protein Kinase 1/biosynthes;" |
| Notes: | "MedlineCandelori, Annalisa Luporini, Pierangelo Alimenti, Claudio Vallesi, Adriana eng Research Support, Non-U.S. Gov't Switzerland 2013/04/05 Int J Mol Sci. 2013 Apr 3; 14(4):7457-67. doi: 10.3390/ijms14047457" |
