| Title: | Investigation of volatile metabolites during growth of Escherichia coli and Pseudomonas aeruginosa by needle trap-GC-MS |
| Author(s): | Zscheppank C; Wiegand HL; Lenzen C; Wingender J; Telgheder U; |
| Address: | "Instrumental Analytical Chemistry, University of Duisburg-Essen, Universitatsstrasse 5, 45141, Essen, Germany" |
| DOI: | 10.1007/s00216-014-8111-2 |
| ISSN/ISBN: | 1618-2650 (Electronic) 1618-2642 (Linking) |
| Abstract: | "A new method for the growth-dependent headspace analysis of bacterial cultures by needle trap (NT)-gas chromatography-mass spectrometry (GC-MS) was established. NTs were used for the first time as enrichment technique for volatile organic compounds (VOCs) in the headspace of laboratory cultures. Reference strains of Escherichia coli and Pseudomonas aeruginosa were grown in different liquid culture media for 48 h at 36 degrees C. In the course of growth, bacterial culture headspace was analysed by NT-GC-MS. In parallel, the abiotic release of volatile organic compounds (VOC) from nutrient media was investigated by the same method. By examination of microbial headspace samples in comparison with those of uninoculated media, it could be clearly differentiated between products and compounds which serve as substrates. Specific microbial metabolites were detected and quantified during the stationary growth phase. P. aeruginosa produced dimethyl sulfide (max. 125 mug L(-1) < limits of quantification (LOQ)), 1-undecene (max. 164 mug L(-1)) and 2-nonanone (max. 200 mug L(-1)), whereas E. coli produced carbon disulfide, butanal and indole (max. 149 mg L(-1)). Both organisms produced isoprene" |
| Keywords: | Equipment Design Escherichia coli/*growth & development/metabolism Gas Chromatography-Mass Spectrometry/*instrumentation Ketones/analysis/metabolism Limit of Detection Pseudomonas aeruginosa/*growth & development/metabolism Sulfides/analysis/metabolism Vo; |
| Notes: | "MedlineZscheppank, C Wiegand, H L Lenzen, C Wingender, J Telgheder, U eng Evaluation Study Germany 2014/08/26 Anal Bioanal Chem. 2014 Oct; 406(26):6617-28. doi: 10.1007/s00216-014-8111-2. Epub 2014 Aug 22" |
