| Title: | The Functional Characterization of Carboxylesterases Involved in the Degradation of Volatile Esters Produced in Strawberry Fruits |
| Author(s): | Zhang L; Zhou K; Wang M; Li R; Dai X; Liu Y; Jiang X; Xia T; Gao L; |
| Address: | "School of Life Sciences, Anhui Agricultural University, Hefei 230036, China. State Key Laboratory of Tea Plant Biology and Utilization, Anhui Agricultural University, Hefei 230036, China. College of Tea Science, Guizhou University, Guiyang 550025, China" |
| ISSN/ISBN: | 1422-0067 (Electronic) 1422-0067 (Linking) |
| Abstract: | "Volatile ester compounds are important contributors to the flavor of strawberry, which affect consumer preference. Here, the GC-MS results showed that volatile esters are the basic aroma components of strawberry, banana, apple, pear, and peach, and the volatile esters were significantly accumulated with the maturation of strawberry fruits. The main purpose of this study is to discuss the relationship between carboxylesterases (CXEs) and the accumulation of volatile ester components in strawberries. FaCXE2 and FaCXE3 were found to have the activity of hydrolyzing hexyl acetate, Z-3-hexenyl acetate, and E-2-hexenyl acetate to the corresponding alcohols. The enzyme kinetics results showed that FaCXE3 had the higher affinity for hexyl acetate, E-2-hexenyl acetate, and Z-3-hexenyl acetate compared with FaCXE2. The volatile esters were mainly accumulated at the maturity stages in strawberry fruits, less at the early stages, and the least during the following maturation stages. The expression of FaCXE2 gradually increased with fruit ripening and the expression level of FaCXE3 showed a decreasing trend, which suggested the complexity of the true function of CXEs. The transient expression of FaCXE2 and FaCXE3 genes in strawberry fruits resulted in a significantly decreased content of volatile esters, such as Z-3-hexenyl acetate, methyl hexanoate, methyl butyrate, and other volatile esters. Taken together, FaCXE2 and FaCXE3 are indeed involved in the regulation of the synthesis and degradation of strawberry volatile esters" |
| Keywords: | Fruit/genetics/metabolism *Fragaria/metabolism Carboxylic Ester Hydrolases/genetics/metabolism Esters/metabolism Acetates/metabolism *Volatile Organic Compounds/metabolism FaCXEs carboxylesterases strawberry volatile ester; |
| Notes: | "MedlineZhang, Lingjie Zhou, Kang Wang, Maohao Li, Rui Dai, Xinlong Liu, Yajun Jiang, Xiaolan Xia, Tao Gao, Liping eng 31870676/Natural Science Foundation of China/ 32072621/Natural Science Foundation of China/ U21A20232/Natural Science Foundation of China/ (2018YFD1000601)/National Key Research and Development Program of China/ Switzerland 2023/01/09 Int J Mol Sci. 2022 Dec 26; 24(1):383. doi: 10.3390/ijms24010383" |
