| Title: | CRISPR/Cas9-based mutation reveals Argonaute 1 is essential for pigmentation in Ostrinia furnacalis |
| Author(s): | You L; Bi HL; Wang YH; Li XW; Chen XE; Li ZQ; |
| Address: | "CAS Key Laboratory of Insect Developmental and Evolutionary Biology, CAS Center for Excellence in Molecular Plant Sciences, Shanghai Institute of Plant Physiology and Ecology, Chinese Academy of Sciences, Shanghai, China" |
| ISSN/ISBN: | 1744-7917 (Electronic) 1672-9609 (Linking) |
| Abstract: | "Ostrinia furnacalis (Lepidoptera: Pyralidae) is one of the most destructive agricultural pests in Asia. Traditional pest-management methods include sex pheromone capture, transgenic crops that produce Bacillus thuringiensis toxin, and pesticides. Although these strategies control pest populations effectively, they also cause negative side effects, including dramatically increased pesticide resistance, severe pollution, and hazards for human health. Recently developed genome editing tools provide new prospects for pest management and have been successfully used in several species. However, few examples have been reported in the agricultural pest O. furnacalis due to a lack in genomic information. In this report, we identified only one transcript of O. furnacalis Argonaute 1 (OfAgo1) gene from the genome and cloned the open reading frame. OfAgo1 presented the maximum expression at the embryo stage or in the fat body during the larval stages. To understand its function, an OfAgo1 mutant was constructed using the Clustered Regularly Interspaced Short Palindromic Repeat/RNA-guided Cas9 nuclease (CRISPR/Cas9). Mutagenesis of OfAgo1 disrupted cuticle pigmentation by down-regulating micro RNAs and pigmentation-related genes. This is the first report for the cloning and functional analysis of OfAgo1, revealing a role of OfAgo1 in cuticle pigmentation. The current report also established a CRISPR/Cas9 system in O. furnacalis, providing a new insight for pest management" |
| Keywords: | "Amino Acid Sequence Animals Argonaute Proteins/*genetics Base Sequence CRISPR-Cas Systems Cloning, Molecular Moths/*genetics Mutation Pigmentation/*genetics Ago1 CRISPR/Cas9 Ostrinia furnacalis cuticle pigmentation pest management;" |
| Notes: | "MedlineYou, Lang Bi, Hong-Lun Wang, Yao-Hui Li, Xiao-Wei Chen, Xi-En Li, Zhi-Qian eng 31372257/National Natural Science Foundation of China/ 31420103918/National Natural Science Foundation of China/ 31601903/National Natural Science Foundation of China/ Australia 2018/06/26 Insect Sci. 2019 Dec; 26(6):1020-1028. doi: 10.1111/1744-7917.12628. Epub 2018 Aug 9" |
