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J Chromatogr B Analyt Technol Biomed Life Sci

Title:		"Development and validation of an HPLC-MS/MS method for the simultaneous analysis of volatile organic compound metabolites, hydroxylated polycyclic aromatic hydrocarbons, and 8-hydroxy-2'-deoxyguanosine in human urine"
Author(s):		Xu RF; Mei H; Chen L; Tang B; Lu QY; Cai FS; Yan X; Zheng J; Shen XT; Yu YJ;
Address:		"State Environmental Protection Key Laboratory of Environmental Pollution Health Risk Assessment, Research Group of Emerging Contaminants, South China Institute of Environmental Sciences, Ministry of Ecology and Environment, Guangzhou 510655, PR China. State Environmental Protection Key Laboratory of Environmental Pollution Health Risk Assessment, Research Group of Emerging Contaminants, South China Institute of Environmental Sciences, Ministry of Ecology and Environment, Guangzhou 510655, PR China; Department of Disease Control and Prevention, General Hospital of Central Theater Command, Wuhan 430030, PR China; State Key Laboratory of Environment Health (Incubation), Key Laboratory of Environment and Health, Ministry of Education, Key Laboratory of Environment and Health (Wuhan), Ministry of Environmental Protection, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, PR China. State Key Laboratory of Environment Health (Incubation), Key Laboratory of Environment and Health, Ministry of Education, Key Laboratory of Environment and Health (Wuhan), Ministry of Environmental Protection, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, PR China. State Environmental Protection Key Laboratory of Environmental Pollution Health Risk Assessment, Research Group of Emerging Contaminants, South China Institute of Environmental Sciences, Ministry of Ecology and Environment, Guangzhou 510655, PR China. Electronic address: tangbin@scies.org. State Environmental Protection Key Laboratory of Environmental Pollution Health Risk Assessment, Research Group of Emerging Contaminants, South China Institute of Environmental Sciences, Ministry of Ecology and Environment, Guangzhou 510655, PR China; School of Public Health, Key Laboratory of Environmental Pollution and Disease Monitoring of Ministry of Education, Guizhou Medical University, Guiyang 550000, PR China"
Journal Title:		J Chromatogr B Analyt Technol Biomed Life Sci
Year:		2023
Volume:		20230914
Issue:
Page Number:		123885 -
DOI:		10.1016/j.jchromb.2023.123885
ISSN/ISBN:		1873-376X (Electronic) 1570-0232 (Linking)
Abstract:		"Humans are widely and concurrently exposed to volatile organic compounds (VOCs) and polycyclic aromatic hydrocarbons (PAHs). However, few studies have reported the internal co-exposure levels of these chemicals in occupational and general populations. Specifically, the associations revealed between the urinary levels of metabolites of VOCs (mVOCs), hydroxylated PAHs (OH-PAHs), and oxidative stress biomarkers for humans remain limited. In this study, a method based on solid-phase extraction (SPE) and liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) was developed for the simultaneous analysis of 22 mVOCs, 12 OH-PAHs, and 8-hydroxy-2'-deoxyguanosine (8-OHdG) in human urine samples. The method was validated with all target analyte accuracies and precisions in the range of 76 %-120 % and 1 %-14 % at three levels of spiked urine samples, respectively. The limit of detection (LOD) and limit of quantification (LOQ) of the target analytes were 0.01-0.34 ng/mL and 0.01-7.57 ng/mL, respectively. And the method was applied to measure urinary levels of target analytes from 38 petrochemical workers in Guangzhou, South China. Except for 3-hydroxy-benzo[a]pyrene, all target analytes were detected in the urine samples. The average levels were 0.05-12.6 ng/mL for individual OH-PAHs, 0.20-73620 ng/mL for individual mVOCs, and 1.00 ng/mL for 8-OHdG. Additionally, 3-hydroxy-phenanthrene, 1-hydroxy-pyrene, 6-hydroxy-chrysene, N-acetyl-S-(trichlorovinyl)-L-cysteine, 2-methylhippuric acid, thiodiacetic acid, trans, trans-Muconic acid, and N-acetyl-S-(3,4-dihydroxybutyl)-L-cysteine had statistically significant positive effects on 8-OHdG levels, while 1-hydroxy-naphthalene, 1,2-dihydroxybenzene, and hippuric acid showed a negative effect on 8-OHdG, indicating these metabolites could lead to synergistic or antagonistic oxidative DNA damage. This study provides a robust analytical method that permits a comprehensive assessment of co-exposure to PAHs and VOCs and their potential adverse health effects"
Keywords:		Biomarkers Hydroxylated polycyclic aromatic hydrocarbons (OH-PAHs) Metabolites of volatile organic compounds (mVOCs) Oxidative stress Urine;
Notes:		"PublisherXu, Rong-Fa Mei, Hang Chen, Li Tang, Bin Lu, Qi-Yuan Cai, Feng-Shan Yan, Xiao Zheng, Jing Shen, Xian-Tao Yu, Yun-Jiang eng Netherlands 2023/09/19 J Chromatogr B Analyt Technol Biomed Life Sci. 2023 Sep 14; 1229:123885. doi: 10.1016/j.jchromb.2023.123885"