| Title: | Large number of putative chemoreception and pheromone biosynthesis genes revealed by analyzing transcriptome from ovipositor-pheromone glands of Chilo suppressalis |
| Author(s): | Xia YH; Zhang YN; Hou XQ; Li F; Dong SL; |
| Address: | "Education Ministry Key Laboratory of Integrated Management of Crop Diseases and Pests, College of Plant Protection, Nanjing Agricultural University, Nanjing, China. 1] Education Ministry Key Laboratory of Integrated Management of Crop Diseases and Pests, College of Plant Protection, Nanjing Agricultural University, Nanjing, China [2] College of Life Sciences, Huaibei Normal University, Huaibei, China" |
| ISSN/ISBN: | 2045-2322 (Electronic) 2045-2322 (Linking) |
| Abstract: | "The chemoreception role of moth ovipositor has long been suggested, but its molecular mechanism is mostly unknown. By transcriptomic analysis of the female ovipositor-pheromone glands (OV-PG) of Chilo suppressalis, we obtained 31 putative chemoreception genes (9 OBPs, 10 CSPs, 2 ORs, 1 SNMP, 8 CXEs and 1 AOX), in addition to 32 genes related to sex pheromone biosynthesis (1 FAS, 6 Dess, 10 FARs, 2 ACOs, 1 ACC, 4 FATPs, 3 ACBPs and 5 ELOs). Tissue expression profiles further revealed that CsupCSP2 and CsupCSP10 were OV-PG biased, while most chemoreception genes were highly and preferably expressed in antennae. This suggests that OV-PG employs mostly the same chemoreception proteins as in antennae, although the physiological roles of these proteins might be different in OV-PG. Of the 32 pheromone biosynthesis related genes, CsupDes4, CsupDes5 and CsupFAR2 are strongly OV-PG biased, and clustered with functionally validated genes from other moths, strongly indicating their involvement in specific step of the pheromone biosynthesis. Our study for the first time identified a large number of putative chemoreception genes, and provided an important basis for exploring the chemoreception mechanisms of OV-PG in C. suppressalis, as well as other moth species" |
| Keywords: | "Animals Gene Expression Profiling Gene Expression Regulation, Developmental Insect Proteins/biosynthesis/*genetics/isolation & purification Lepidoptera/*genetics Oviposition/genetics Pheromones/biosynthesis/*genetics/isolation & purification Transcriptome;" |
| Notes: | "MedlineXia, Yi-Han Zhang, Ya-Nan Hou, Xiao-Qing Li, Fei Dong, Shuang-Lin eng Research Support, Non-U.S. Gov't England 2015/01/21 Sci Rep. 2015 Jan 20; 5:7888. doi: 10.1038/srep07888" |
