| Title: | Exposure of Helicoverpa armigera Larvae to Plant Volatile Organic Compounds Induces Cytochrome P450 Monooxygenases and Enhances Larval Tolerance to the Insecticide Methomyl |
| Author(s): | Wu C; Ding C; Chen S; Wu X; Zhang L; Song Y; Li W; Zeng R; |
| Address: | "Key Laboratory of Vector Biology and Pathogen Control of Zhejiang Province, School of Life Sciences, Huzhou University, Huzhou 313000, China. Guangdong Provincial Key Laboratory of Crop Genetic Improvement, Crops Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China. State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, College of Agriculture, Fujian Agriculture and Forestry University, Jinshan, Fuzhou 350002, China. College of Materials and Energy, South China Agricultural University, Wushan, Guangzhou 510642, China" |
| ISSN/ISBN: | 2075-4450 (Print) 2075-4450 (Electronic) 2075-4450 (Linking) |
| Abstract: | "Plants release an array of volatile chemicals into the air to communicate with other organisms in the environment. Insect attack triggers emission of herbivore-induced plant volatiles (HIPVs). How insect herbivores use these odors to plan their detoxification systems is vital for insect adaptation to environmental xenobiotics. Here we show that the larvae of Helicoverpa armigera (Hubner), a broadly polyphagous lepidopteran herbivore, have the capacity to use plant volatiles as cues to upregulate multiple detoxification systems, including cytochrome P450 monooxygenases (P450s), for detoxification of insecticides. Olfactory exposure of the fifth instars to two terpene volatiles limonene and nerolidol, and two green-leaf volatiles 2-heptanone and cis-3-hexenyl acetate significantly reduced larval susceptibility to the insecticide methomyl. However, larval pretreatment with piperonyl butoxide (PBO), a known P450 inhibitor, neutralized the effects of volatile exposure. Furthermore, larval exposure to the four plant volatiles enhanced activities of P450 enzymes in midguts and fatbodies, and upregulated expression of CYP6B2, CYP6B6 and CYP6B7, P450s involved in detoxification of the insecticide. Larval exposure to 2-heptanone and limonene volatiles also enhanced activities of glutathione-s-transferase and carboxylesterase. Our findings suggest that olfactory exposure to HIPVs enhances larval insecticide tolerance via induction of detoxification P450s" |
| Keywords: | Helicoverpa armigera detoxification enzyme herbivore-induced plant volatiles cytochrome P450 monooxygenases insecticide tolerance plant volatile; |
| Notes: | "PubMed-not-MEDLINEWu, Choufei Ding, Chaohui Chen, Shi Wu, Xiaoying Zhang, Liqin Song, Yuanyuan Li, Wu Zeng, Rensen eng 31870361/National Natural Science Foundation of China/ 2020J02030/Natural Science Foundation of Fujian Province/ Switzerland 2021/04/04 Insects. 2021 Mar 12; 12(3):238. doi: 10.3390/insects12030238" |
