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Mol Cell Biol

Title:		The Mre11-Rad50-Xrs2 protein complex facilitates homologous recombination-based double-strand break repair in Saccharomyces cerevisiae
Author(s):		Bressan DA; Baxter BK; Petrini JH;
Address:		"Laboratory of Genetics, University of Wisconsin Medical School, Madison, Wisconsin 53706, USA"
Journal Title:		Mol Cell Biol
Year:		1999
Volume:		19
Issue:		11
Page Number:		7681 - 7687
DOI:		10.1128/MCB.19.11.7681
ISSN/ISBN:		0270-7306 (Print) 1098-5549 (Electronic) 0270-7306 (Linking)
Abstract:		"Saccharomyces cerevisiae mre11Delta mutants are profoundly deficient in double-strand break (DSB) repair, indicating that the Mre11-Rad50-Xrs2 protein complex plays a central role in the cellular response to DNA DSBs. In this study, we examined the role of the complex in homologous recombination, the primary mode of DSB repair in yeast. We measured survival in synchronous cultures following irradiation and scored sister chromatid and interhomologue recombination genetically. mre11Delta strains were profoundly sensitive to ionizing radiation (IR) throughout the cell cycle. Mutant strains exhibited decreased frequencies of IR-induced sister chromatid and interhomologue recombination, indicating a general deficiency in homologous recombination-based DSB repair. Since a nuclease-deficient mre11 mutant was not impaired in these assays, it appears that the role of the S. cerevisiae Mre11-Rad50-Xrs2 protein complex in facilitating homologous recombination is independent of its nuclease activities"
Keywords:		Animals *DNA Repair *DNA-Binding Proteins *Endodeoxyribonucleases *Exodeoxyribonucleases Fungal Proteins/genetics/*metabolism Gamma Rays Gene Deletion Interphase Macromolecular Substances Mating Factor Mutation Peptides/pharmacology Radiation Tolerance *R;
Notes:		"MedlineBressan, D A Baxter, B K Petrini, J H eng T32 CA009471/CA/NCI NIH HHS/ T32-CA09471/CA/NCI NIH HHS/ 5T32GM07133/GM/NIGMS NIH HHS/ GM56888/GM/NIGMS NIH HHS/ T32 GM007133/GM/NIGMS NIH HHS/ R01 GM056888/GM/NIGMS NIH HHS/ Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. 1999/10/19 Mol Cell Biol. 1999 Nov; 19(11):7681-7. doi: 10.1128/MCB.19.11.7681"