| Title: | "Localization of aggregation substances of Enterococcus faecalis after induction by sex pheromones. An ultrastructural comparison using immuno labelling, transmission and high resolution scanning electron microscopic techniques" |
| Author(s): | Wanner G; Formanek H; Galli D; Wirth R; |
| Address: | "Botanisches Institut, Universitat Munchen, Federal Republic of Germany" |
| ISSN/ISBN: | 0302-8933 (Print) 0302-8933 (Linking) |
| Abstract: | "The distribution of sex pheromone induced aggregation substance was studied on the cell surface of various Enterococcus faecalis strains. In the accompanying paper we have shown that the aggregation substance appears as a layer of hairlike structures. Using direct and indirect immunogold technique, transmission electron microscopy and high resolution scanning electron microscopy we investigated the appearance and distribution of the aggregation substance. The 'hairs' increase in number with increasing exposure to sex pheromones (maximum density: 1300/microns2). We show that these structures are unequally distributed over the cell surface, even if the cells were induced by sex pheromones for a long period of time. Statistical analysis of the unequal distribution indicates that aggregation substance is incorporated into pre-existing 'old' cell-walls and that this incorporation shows a saturation ca. 40 min after addition of sex pheromones" |
| Keywords: | "Biological Factors/*analysis/biosynthesis Cell Wall/analysis Enterococcus faecalis/analysis/*metabolism/ultrastructure Immunohistochemistry Microscopy, Electron Microscopy, Electron, Scanning Pheromones/*metabolism Sex Attractants/*metabolism;" |
| Notes: | "MedlineWanner, G Formanek, H Galli, D Wirth, R eng Research Support, Non-U.S. Gov't Germany 1989/01/01 Arch Microbiol. 1989; 151(6):491-7. doi: 10.1007/BF00454864" |
