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J Pharm Biomed Anal

Title:		Determination of volatile organic compounds in SW620 colorectal cancer cells and tumor-bearing mice
Author(s):		Wang G; Li Y; Liu M; Guo N; Han C; Liu D; Li D; Yang M; Peng Y; Liu Y; Yu K; Wang C;
Address:		"Department of Critical Care Medicine, Harbin Medical University Cancer Hospital, Harbin, China. Department of Critical Care Medicine, the First Affiliated Hospital of Harbin Medical University, Harbin, China. Department of Critical Care Medicine, Harbin Medical University Cancer Hospital, Harbin, China. Electronic address: drkaijiang@163.com. Department of Critical Care Medicine, Harbin Medical University Cancer Hospital, Harbin, China. Electronic address: changsongwangicu@163.com"
Journal Title:		J Pharm Biomed Anal
Year:		2019
Volume:		20190130
Issue:
Page Number:		30 - 37
DOI:		10.1016/j.jpba.2019.01.050
ISSN/ISBN:		1873-264X (Electronic) 0731-7085 (Linking)
Abstract:		"Early diagnosis and early treatment are important factors in reducing colorectal cancer (CRC) metastasis and mortality. Volatile organic compounds (VOCs) released by the human body have great potential for use in clinical diagnosis and therapeutic monitoring for CRC. The aim of our study was to identify VOCs with high specificity and high sensitivity for CRC and to provide a method for early diagnosis of CRC. Gas chromatography-mass spectrometry (GC-MS) was utilized to analyze metabolites in both the in vivo and in vitro experimental groups. In vivo, VOCs were analyzed in the blood of mice after cell inoculation and tumor resection. In vitro experiments were performed by comparing changes in VOCs in an HCoEpiC cell group, control group, SW620 cell group and Arsenic trioxide + SW620 group. We observed changes in VOCs in a series of CRC SW620 cells in vivo and in vitro. Among these changes, we found that the concentrations of 8 substances, including acetone, increased with tumor growth. Nine substances were found to be significantly elevated in the SW620 cancer cell group compared with the other groups. Only one substance was consumed by the tumor in both the in vivo and in vitro experiments. Our study showed that alkanes, lipids, alcohols, ketones, aldehyde, butylated hydroxytoluene (BHT) and hexamethylcyclotrisiloxane all existed at different levels in SW620 CRC cells compared to those in normal cells. We need more research to further confirm this hypothesis"
Keywords:		"Animals Arsenates Biomarkers, Tumor/*analysis/blood Cell Line, Tumor Colorectal Neoplasms/blood/*metabolism/pathology Gas Chromatography-Mass Spectrometry Humans Mice Mice, Nude Neoplasm Transplantation Solid Phase Microextraction Volatile Organic Compoun;"
Notes:		"MedlineWang, Guiyue Li, Yuhang Liu, Miao Guo, Nana Han, Ci Liu, Desheng Li, Dandan Yang, Mengyuan Peng, Yahui Liu, Yansong Yu, Kaijiang Wang, Changsong eng England 2019/02/10 J Pharm Biomed Anal. 2019 Apr 15; 167:30-37. doi: 10.1016/j.jpba.2019.01.050. Epub 2019 Jan 30"