| Title: | Key Residues Involved in the Interaction between Cydia pomonella Pheromone Binding Protein 1 (CpomPBP1) and Codlemone |
| Address: | "Key Laboratory of Plant Protection Resources & Pest Management of the Ministry of Education, College of Plant Protection, Northwest A&F University , Yangling 712100, Shaanxi, China" |
| ISSN/ISBN: | 1520-5118 (Electronic) 0021-8561 (Linking) |
| Abstract: | "Codlemone exhibited high affinity to CpomPBP1; studying their binding mode can provide insights into the rational design of active semiochemicals. Our findings suggested that residues including Phe12, Phe36, Trp37, Ile52, Ile 94, Ala115, and Phe118 were favorable to the binding of codlemone to CpomPBP1, whereas residues providing unfavorable contributions such as Ser56 were negative to the binding. van der Waals energy and electrostatic energy, mainly derived from the side chains of favorable residues, contributed most to the formation and stability of the CpomPBP1-codlemone complex. Of the residues involved in the interaction between CpomPBP1 and codlemone, Phe12 and Trp37, the mutation of which into Ala caused a significant decrease of CpomPBP1 binding ability, were two key residues in determining the binding affinity of codlemone to CpomPBP1. This study shed light on discovering novel active semiochemicals as well as facilitating chemical modification of lead semiochemicals" |
| Keywords: | CpomPBP1 binding free energy decomposition codlemone computational alanine scanning site-directed mutagenesis; |
| Notes: | "PubMed-not-MEDLINETian, Zhen Liu, Jiyuan Zhang, Yalin eng 2016/10/07 J Agric Food Chem. 2016 Oct 26; 64(42):7994-8001. doi: 10.1021/acs.jafc.6b02843. Epub 2016 Oct 17" |
