| Title: | "The Farnesyltransferase beta-Subunit Ram1 Regulates Sporisorium scitamineum Mating, Pathogenicity and Cell Wall Integrity" |
| Author(s): | Sun S; Deng Y; Cai E; Yan M; Li L; Chen B; Chang C; Jiang Z; |
| Address: | "Guangdong Province Key Laboratory of Microbial Signals and Disease Control, College of Agriculture, South China Agricultural University, Guangzhou, China. Sugarcane Research Institute, Guangxi Academy of Agricultural Sciences, Nanning, China. State Key Laboratory of Conservation and Utilization of Subtropical Agro-Bioresources, Guangxi University, Nanning, China" |
| ISSN/ISBN: | 1664-302X (Print) 1664-302X (Electronic) 1664-302X (Linking) |
| Abstract: | "The basidiomycetous fungus Sporisorium scitamineum causes a serious sugarcane smut disease in major sugarcane growing areas. Sexual mating is essential for infection to the host; however, its underlying molecular mechanism has not been fully studied. In this study, we identified a conserved farnesyltransferase (FTase) beta subunit Ram1 in S. scitamineum. The ram1Delta mutant displayed significantly reduced mating/filamentation, thus of weak pathogenicity to the host cane. The ram1Delta mutant sporidia showed more tolerant toward cell wall stressor Congo red compared to that of the wild-type. Transcriptional profiling showed that Congo red treatment resulted in notable up-regulation of the core genes involving in cell wall integrity pathway in ram1Delta sporidia compared with that of WT, indicating that Ram1 may be involved in cell wall integrity regulation. In yeast the heterodimeric FTase is responsible for post-translational modification of Ras (small G protein) and a-factor (pheromone). We also identified and characterized two conserved Ras proteins, Ras1 and Ras2, respectively, and a MAT-1 pheromone precursor Mfa1. The ras1Delta, ras2Delta and mfa1Delta mutants all displayed reduced mating/filamentation similar as the ram1Delta mutant. However, both ras1Delta and ras2Delta mutants were hypersensitive to Congo red while the mfa1Delta mutant was the same as wild-type. Overall our study displayed that RAM1 plays an essential role in S. scitamineum mating/filamentation, pathogenicity, and cell wall stability" |
| Keywords: | Ram1 Ras Sporisorium scitamineum cell wall integrity mating; |
| Notes: | "PubMed-not-MEDLINESun, Shuquan Deng, Yizhen Cai, Enping Yan, Meixin Li, Lingyu Chen, Baoshan Chang, Changqing Jiang, Zide eng Switzerland 2019/05/28 Front Microbiol. 2019 May 8; 10:976. doi: 10.3389/fmicb.2019.00976. eCollection 2019" |
