| Title: | Characterization of kefir yeasts with antifungal capacity against Aspergillus species |
| Author(s): | Moure MC; Perez Torrado R; Garmendia G; Vero S; Querol A; Alconada T; Leon Pelaez A; |
| Address: | "Centro de Investigacion y Desarrollo en Fermentaciones Industriales (CINDEFI), UNLP, CCT?ªªLa Plata, CONICET, Facultad de Ciencias Exactas, Universidad Nacional de La Plata, Calle 47 y 115, B1900ASH, La Plata, Buenos Aires, Argentina. candelamoure1@gmail.com. Catedra de Microbiologia-Facultad de Ciencias Exactas, Universidad Nacional de La Plata, Calle 47 y 115, B1900ASH, La Plata, Buenos Aires, Argentina. candelamoure1@gmail.com. Instituto de Agroquimica y Tecnologia de Alimentos (IATA)-CSIC, Avda. Catedratico Agustin Escardino, 7, 46980, Paterna, Valencia, Spain. Facultad de Quimica, Catedra de Microbiologia, Universidad de la Republica, Gral. Flores 2124, 11800, Montevideo, Uruguay. Centro de Investigacion y Desarrollo en Fermentaciones Industriales (CINDEFI), UNLP, CCT?ªªLa Plata, CONICET, Facultad de Ciencias Exactas, Universidad Nacional de La Plata, Calle 47 y 115, B1900ASH, La Plata, Buenos Aires, Argentina. Catedra de Microbiologia-Facultad de Ciencias Exactas, Universidad Nacional de La Plata, Calle 47 y 115, B1900ASH, La Plata, Buenos Aires, Argentina" |
| DOI: | 10.1007/s10123-022-00296-z |
| ISSN/ISBN: | 1618-1905 (Electronic) 1139-6709 (Linking) |
| Abstract: | "Kefir is a fermented probiotic drink obtained by placing kefir granules in a suitable substrate. The kefir granules are a consortium of bacteria and yeasts embedded in a exopolysaccharide matrix. The aim of this research was the isolation and identification of yeasts from kefir of different origin, the evaluation of their antifungal capacity against Aspergillus spp., and the characterization of virulence related traits. Using RFLP of ITS1/ITS4 region, D1/D2 region sequencing, and RAPD techniques, 20 kefir isolates were identified as Geotrichum candidum, Pichia kudriavzevii, Pichia membranifaciens, Saccharomyces cerevisiae, and Candida ethanolica. Their antifungal capacity was evaluated by their conidia germination reduction, which allowed the selection of eight isolates with high to moderate conidia germination reduction against Aspergillus flavus and Aspergillus parasiticus. Furthermore, these selected isolates showed growth inhibition on contact in the dual culture assay for both Aspergillus species and 3 of them-belonging to S. cerevisiae and P. kudriavzevii species-generated volatile organic compounds which significantly affected the growth of both fungi. For the evaluation of virulence-related traits, growth at high temperatures, enzymatic activities, and the adhesion to Caco-2 cells were analyzed. The isolates did not present more than one positive virulence-related trait simultaneously. In particular, it is important to highlight that the adhesion capacity to the model of intestinal barrier was extremely low for all of them. According to the results obtained, further studies would be of interest for the possible use of these promising yeasts as biocontrol agents against fungi in food" |
| Keywords: | Humans *Antifungal Agents/pharmacology *Kefir Saccharomyces cerevisiae/genetics Random Amplified Polymorphic DNA Technique Caco-2 Cells Yeasts/genetics Aspergillus Antifungal capacity Aspergillus spp.Contaminant fungi Kefir yeasts Virulence-related trait; |
| Notes: | "MedlineMoure, Maria Candela Perez Torrado, Roberto Garmendia, Gabriela Vero, Silvana Querol, Amparo Alconada, Teresa Leon Pelaez, Angela eng PIP: 0819/Consejo Nacional de Investigaciones Cientificas y Tecnicas/ 11x838/Universidad Nacional de La Plata/ PICT 2018 03702/Agencia Nacional de Promocion Cientifica y Tecnologica/ Switzerland 2022/11/13 Int Microbiol. 2023 May; 26(2):361-370. doi: 10.1007/s10123-022-00296-z. Epub 2022 Nov 12" |
